REV感染DF-1细胞分泌外体的生物信息学分析  

Bioinformatics Analyses of Exosome from REV-Infected DF-1 Cells

作　　者：王小满[1,2] 庄萍萍 孙霞[3] 杜旭升 王晓宇 成子强 王桂花[1,2] 

机构地区：[1]山东农业大学动物科技学院,泰安271018 [2]山东省动物生物功能与疾病防治重点实验室,泰安271018 [3]山东畜牧兽医职业学院,潍坊261061

出　　处：《中国细胞生物学学报》2017年第12期1565-1572,共8页Chinese Journal of Cell Biology

基　　金：山东省自然基金(批准号:ZR2017MC011);山东省中青年科学家奖励基金(批准号:BS2013SW016);山东省"双一流"奖补资金资助的课题~~

摘　　要：该研究探讨了REV感染DF-1细胞分泌外体携带表达差异的蛋白质和微RNA(micro RNA,mi RNA),及其基因功能和参与的信号通路,为病毒致病机制研究提供了基础。通过蛋白组学和转录组学检测技术,筛选病毒感染组与对照组DF-1细胞分泌外体的差异蛋白质和mi RNA,并利用在线软件数据库对其进行GO功能富集和KEGG信号通路分析。结果筛选到101个差异表达蛋白质,其中56个表达上调,45个表达下调,共参与155条信号通路,参与蛋白质最多的是肿瘤相关通路;并筛选到3个表达上调的mi RNA,其中mi RNA-155、mi RNA-146a-3p编码的靶蛋白(整合蛋白)与差异蛋白质肌动蛋白相关2/3复合体(actinrelated 2/3 complexs,Arp2/3)共同参与肌动蛋白细胞骨架信号通路;差异蛋白质及mi RNA编码靶基因均含有病毒成分,并参与细胞信号转导、免疫、黏附、运动、生物调节等过程。该研究结果表明,REV感染DF-1细胞来源外体表达差异的蛋白质和mi RNA及其参与的生物学过程和信号通路与肿瘤形成密切相关,外体途径可能在REV致瘤机制中具有重要作用。The study was to identify the differentially expressed proteins and micro RNA（mi RNA）, their gene function and involved signal pathway of exosome from REV-infected DF-1 cells. The differential proteins and mi RNA of exosome were screened between the virus infection group and control group by proteomics and transcription detection technology, and then analyzed with GO function enrichment and KEGG signal pathway database. The results demonstrated that 101 different expressed proteins were screened with 56 up-regulated and 45 down-regulated. These proteins were involved in 155 signal pathways, and the number of proteins involved in the cancer pathway was the highest. There was 3 up-regulated mi RNA screened. Both the target proteins（integrins） of mi RNA-155 and mi RNA-146 a-3 p, and the differential protein actinrelated 2/3 complexs（Arp2/3） participated in actin cytoskeleton signal pathway. Otherwise, they contained viral components, and involved in cell signaling, immunization, adhesion, locomotion, biological regulation and other processes. These results suggested that the differentially expressed proteins and mi RNA, their gene function and involved signal pathway of exosome from REV infected DF-1 cells were associated with tumor formation. Exosome pathway may play an important role in the REV tumorigenesis mechanism.

关 键 词：REV 外体 肌动蛋白细胞骨架信号通路 致瘤性 

分 类 号：S852.65[农业科学—基础兽医学]