乙型肝炎病毒感染者疾病进展程度与宿主CYP3A5和MDR1基因多态性研究  

The association between the progression of hepatitis B patients and the genetic polymorphisms of CYP3A5 and MDR1

作　　者：杨旭洁 卢永杰[1] 陈捷[1] 吴晓娟[1] 苏真珍[1] 蔡蓓[1] 

机构地区：[1]四川大学华西医院实验医学科,成都610041

出　　处：《检验医学与临床》2018年第1期1-3,8,共4页Laboratory Medicine and Clinic

基　　金：国家自然科学基金资助项目(81401666)

摘　　要：目的探讨细胞色素P450(CYP450)家族CYP3A5和人类多药耐药基因(MDR1)C3435T基因型与乙型肝炎(乙肝)患者病程进展的关系。方法收集187例慢性乙肝、163例乙肝肝硬化、174例乙肝肝癌患者外周血标本,提取DNA,采用聚合酶链反应扩增及高分辨熔解曲线(HRM)法对患者的CYP3A5和MDR1C3435T基因进行分型。结果 CYP3A5和MDR1C3435T的等位基因分布频率在慢性乙肝组与乙肝肝硬化组、乙肝肝癌组间差异有统计学意义(P<0.05)。结论携带CYP3A5(6986A>G)A基因或MDR1C3435T的T基因的乙肝患者发生疾病进展的风险较高,但仍需进一步扩大样本量验证。Objective To explore the the progression of hepatitis B patients and the genetic polymorphisms of CYP3 A5 and MDR1.Methods The experimental subjects composed of 187 CHB patients,163 hepatitis Brelated cirrhosis,174 hepatitis B-related hepatocelluar carcinomas.The genetic polymorphisms of CYP3 A5 and MDR1 were analyzed by polymerase chain reaction and high resolution melting.Results The genetype of CYP3 A5 and MDR1 was different in hepatitis B and disease progress group,and the difference was statistically significant(P<0.05).Conclusion CYP3 A5 and MDR1 may be related to progressive risk of HBV infection patients.CYP3 A5 Gand MDR1 Care protected genes in hepatitis B-infected patients.

关 键 词：乙型肝炎 肝硬化 肝癌 CYP3A5基因 MDR1基因 

分 类 号：R446.1[医药卫生—诊断学]