50 Hz 1.8 mT正弦交变电磁场对生长期大鼠骨密度的影响研究  

Effect of 50 Hz 1.8 mT sinusoidal electromagnetic fields on bone mineral density in growing rats

作　　者：高玉海[1] 周延峰[1] 李少锋[1] 李文苑 葸慧荣 杨芳芳 陈克明[1] 

机构地区：[1]兰州军区总医院全军创伤骨科研究所,甘肃兰州730050

出　　处：《中国骨伤》2017年第12期1113-1117,共5页China Journal of Orthopaedics and Traumatology

基　　金：国家自然科学基金(编号:81270963);国家国际科技合作专项项目(编号:2015DFR30940);甘肃省空间辐射生物学重点实验室开放基金(编号:2016GKL002)~~

摘　　要：目的 :探讨50 Hz 1.8 mT正弦交变电磁场(sinusoidal electromagnetic fields,SEMFs)对SD大鼠骨密度(bone mineral density,BMD)的影响。方法 :将30只1月龄体重为(110±10)g的SD大鼠随机分为正常对照组和电磁场组,各15只。分别给予对照组50 Hz 0 mT和电磁场组50 Hz 1.8 mT强度正弦交变电磁场干预,1.5 h/d,每周称取体重1次,每天监测进食量变化,6周后腹腔注射麻醉大鼠并用双能X骨密度检测全身骨密度,处死后检测股骨骨密度及椎体骨密度;ELISA法测定血清骨钙素(Osteocalcin,OC)和血清抗酒石酸性磷酸酶5b(tartrate resistant acid phosphatase5b,TRACP 5b);剥离肝、肾、子宫称重,计算器官指数并做HE切片做常规病理学检测分析。结果:与对照组相比,电磁场组大鼠各周体重、每日进食量未见明显变化;2、4周后全身骨密度无明显变化,6周后全身骨密度及离体股骨和椎骨骨密度显著增加;血清OC表达量显著增加,血清TRACP 5b表达量显著降低;子宫、肝脏、脾脏HE染色未见病变,脏器指数无异常。结论:50 Hz 1.8 mT正弦交变电磁场能通过提高骨形成降低骨吸收相关因子的表达,从而提高青年大鼠的峰值骨密度,为临床电磁场预防骨质疏松的研究奠定基础。Objective：To study effects of 50 Hz 1.8 mT sinusoidal electromagnetic fields（SEMFs） on bone mineral density（BMD） in SD rats. Methods：Thirty SD rats weighted（110±10） and aged 1 month were randomly divided into control group and electromagnetic field group,15 in each group. Normal control group of 50 Hz 0 mT density and sinusoidal electromagnetic field group of 50 Hz 1.8 mT were performed respectively with 1.5 h/d and weighted weight once a week,and observed food intake. Rats were anesthesia by intraperitoneal injection and dual energy X-ray absorptiometry were used to detect bone density of whole body,and detected bone density of femur and vertebral body. Osteocalcin and tartrate- resistant acid phosphatase 5 b were detected by ELSA;weighted liver,kidney and uterus to calculate purtenance index,then detected pathologic results by HE. Results：Compared with control group,there was no significant change in weight every week,food-intake every day; no obvious change of bone density of whole body at 2 and 4 weeks,however bone density of whole body,bone density of excised femur and vertebra were increased at 6 weeks. Expression of OC was increased,and TRACP 5 b expression was decreased. No change of HE has been observed in liver,kidney and uterus and organic index. Conclusion：50 Hz 1.8 mT sinusoidal electromagnetic fields could improve bone formation to decrease relevant factors of bone absorbs,to improve peak bone density of young rats,in further provide a basis for clinical research electromagnetic fields preventing osteoporosis foundation.

关 键 词：电磁场 骨密度 大鼠 

分 类 号：R651.15[医药卫生—外科学]