小体积液相萃取-GC/MS法检验尿液中的MDPV  

Determination of MDPV in human urine by liquid-phase small-extraction-GC/MS

作　　者：阎仁信 朱国玉 石建忠 吴卫兵 

机构地区：[1]江苏省南通市公安局物证鉴定所,江苏南通226007

出　　处：《中国法医学杂志》2017年第6期639-641,共3页Chinese Journal of Forensic Medicine

摘　　要：目的建立小体积液相萃取-GC/MS法检验尿液中的MDPV分析方法。方法将添加MDPV标准品的尿液调p H值为9,采用小体积二氯甲烷进行提取,有机相直接进行GC/MS分析。结果 MDPV的线性范围为0.05μg/m L-0.20μg/m L(R2=0.9965),检出限为0.02μg/m L,提取回收率高于85%,精密度小于10%。结论本文建立的方法操作简单、灵敏、快速、经济,适用于吸毒检验工作中尿液中MDPV的检验。Objective The method for the analysis of Methylenedioxypyrovalerone（MDPV） in urine by liquidphase small-extraction-GC/MS have been developed and studied.Methods A 1-m L of urine sample was adjusted to pH9 with sodium bicarbonate,and was extracted with 50μL dichloromethane.The mixture centrifuged at 14000 g for 2 min.A 1-μL organic phase was injected into the GC/MS system.Results The calibration curves showed good linearity in range of 0.05μg/m L^0.20μg/m L,and the limit of detection was 0.02μg/m L,and the RSD was 3.86~5.69%,and the recovery rate was 86.5~92.8 %.Conclusion The method is sensitive,accurate and was easy to operate for fast detection of MDPV in human urine.

关 键 词：法医毒物分析 MDPV 亚甲基二氧吡咯戊酮 尿液 GC/MS 

分 类 号：D919.2[医药卫生—法医学]