血液中血小板与白细胞抗体的固相化筛查  

Solid phase screening of antibodies against platelet and white blood cells in blood

作　　者：纪勇平 徐燕娜 黄萃 周斌 

机构地区：[1]丽水市中心血站,浙江丽水323000

出　　处：《临床血液学杂志（输血与检验）》2017年第6期951-954,共4页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

基　　金：丽水市输血医学重点学科建设项目(No:2013YXZD01)

摘　　要：目的:使用固相化方法检测输血患者体内针对血小板、淋巴细胞与粒细胞抗体的产生情况。方法:收集丽水地区597例输血患者输血前后的血样,使用固相化抗体筛查技术对患者血样进行筛查,分析患者输血之后,体内针对血小板、淋巴细胞与粒细胞的抗体产生频率。结果:使用固相化抗体筛选体系对待检样本进行筛查之后,检出输血前血小板抗体72例(12.06%),淋巴细胞抗体103例(17.25%),粒细胞抗体108例(18.09%),混合抗体(包括同时存在2种或3种抗体)48例(8.04%),输血后血小板抗体102例(17.09%),淋巴细胞抗体105例(17.59%),粒细胞抗体115例(19.26%),混合抗体74例(12.40%)。结论:患者输血后针对血小板和白细胞抗原的抗体产生频率会增加,使用固相化抗体筛查体系可以进行高通量血液抗体筛查。经过抗体筛查可以提高输血安全性。Objective：To detect antibodies against platelets,lymphocytes and neutrophils in the patients received transfusion by solid phase method.Method：A total of 597 patients＇ samples before and after blood transfusion were collected in Lishui area.Antibodies were screened by solid phase method,and the generate frequency of antibodies against platelets,lymphocytes and neutrophils were analyzed.Result：Seventy-two platelet antibodies,103 lymphocyte antibodies,108 neutrophil antibodies and 48 mixed（two or three antibodies against platelets,lymphocytes or neutrophils existing at the same time）antibodies were detected in before transfused samples;and102 platelet antibodies,105 lymphocyte antibodies,115 neutrophil antibodies and 74 mixed antibodies were detected in after transfused samples by solid phase screening.Conclusion：The generate frequency of antibodies against platelets and white blood cells would rise up after transfusion,and high through antibodies screening could be performed by solid phase method.Transfusion safety can improved by antibodies screening.

关 键 词：抗体筛查 固相化 血小板抗体 

分 类 号：R457.1[医药卫生—治疗学]