基于UPLC-Triple TOF-MS/MS技术分析同基源何首乌和首乌藤中差异化学成分  

Analysis of Chemical Constituents in Polygoni Multiflori Radix and Polygoni Multifori Caulis from the Same Origin Based on UPLC-Triple TOF-MS/MS

作　　者：罗益远 蔡中齐[1] 刘训红[2] 蔡伟[1] 刘娟秀[2] 王胜男[2] 华愉教 

机构地区：[1]浙江医药高等专科学校,宁波315100 [2]南京中医药大学,南京210023

出　　处：《天然产物研究与开发》2017年第12期2068-2074,共7页Natural Product Research and Development

基　　金：国家科技支撑计划(2011BAI13B04);江苏高校优势学科建设工程资助项目(ysxk-2014)

摘　　要：基于超高效液相-串联四极杆飞行时间高分辨质谱法(UPLC-Triple TOF-MS/MS)结合多元统计分析技术分析同基源何首乌和首乌藤中化学成分的差异。何首乌样品用甲醇在室温下超声提取后进样,通过二级串联质谱分析,对采集的图谱进行峰匹配、峰对齐、滤噪处理等进行特征峰提取;用主成分分析(PCA)和偏最小二乘法-判别分析(PLS-DA)进行数据处理;根据一级质谱精确质荷比和二级质谱碎片信息,结合软件数据库搜索及相关文献进行成分鉴定。结果显示,同基源何首乌和首乌藤样品间的化学组成得到明显区分;初步筛选并鉴定出42种同基源何首乌和首乌藤差异显著的化学成分,其中12种为共有差异化学成分,且呈现出不同的变化规律。该结果可为揭示同基源何首乌和首乌藤中次生代谢产物合成积累的差异性提供基础资料,亦为何首乌和首乌藤资源的综合开发利用提供了科学依据。The difference of chemical composition in Polygoni Multiflori Radix （PMR） and Polygoni Muhifori Caulis （PMC） from the same origin was analyzed by ultra-performance liquid chromatography coupled with triple quadrupole- time of flight tandem mass spectrometry （ UPLC-Triple TOF-MS/MS）. The samples were extracted with methanol by ul- trasonication at room temperature and centrifuged. Through the analysis of the multistage tandem mass spectrometry, the characteristic peaks were extracted with mass spectrometry data peak matching, peak alignment, noise filtering. The ob- tained data were analyzed by principal component analysis （PCA） and partial least squares-discriminant analysis （ PLS- DA）. The components were identified according to MS accurate mass and MS/MS spectrometry fragmentation informa- tion, combined with the software of database searching and literature component identification. The results showed that the chemical compositions between PMR and PMC from the same origin were clearly distinguished and 42 chemical com- positions were identified. Among of them, 12 compositions were common chemical composition presented in different changing laws. The research provided experimental data for revealing the laws on metabolites of PMR and PMC from the same origin.

关 键 词：何首乌 首乌藤 UPLC-Triple TOF-MS/MS 差异化学成分 

分 类 号：O657.63[理学—分析化学] R284.1[理学—化学]