慢性阻塞性肺疾病对单个核细胞TLR 2/4信号通路的影响  

Influence of chronic obstructive pulmonary disease on the expression of toll-like receptor 2/4 signal pathway of peripheral blood mononuclear cells

作　　者：吴玉英 王晓华[1] 

机构地区：[1]南京医科大学附属常州医院常州市第二人民医院呼吸科,江苏常州213003

出　　处：《实用临床医药杂志》2017年第24期16-18,22,共4页Journal of Clinical Medicine in Practice

摘　　要：目的观察慢性阻塞性肺疾病(COPD)组患者外周血单个核细胞(PBMC)TLR2/TLR4、IRAK and NF-κB mRNA水平的变化。方法利用Ficoll分离法分离健康人及COPD组患者外周血单个核细胞,通过q PCR的方法检测健康人及COPD患者的TLR2/TLR4、IRAK、NF-κB mRNA的表达水平。结果 COPD组单核细胞TLR2、TLR4 mRNA水平较健康人未见明显变化,但IRAK1/4以及NF-κB mRNA水平的变化相对健康对照组显著下降(P<0.05)。结论 TLR2/4信号通路的表达下调参与了COPD疾病进展。Objective To investigate the expression changes of toll-like receptor 2/4 （TLR 2/4）, IRAK and NF-KB mRNA on monocyte-derived dendritic cells of patients with chronic obstruc- tive pulmonary disease. Methods Ficoll separation was been used to separate peripheral blood mononuclear cells of the healthy controls and patients with COPD. The expression levels of TLR2/TLR4, IRAK and NF-κB mRNA were detected for healthy controls and patients with COPD by the method of qPCR. Results The expression of TLR2 and TLR4 mRNA in COPD group were similar to healthy people, but IRAK1/4 and the NF-κB mRNA expression levels were significantly lower than the healthy controls. Conclusion The download expression of TLR2/4 signaling pathways play an important role in the progression of COPD.

关 键 词：慢性阻塞性肺疾病 Toll样受体2/4 IRAK NF-ΚB 

分 类 号：R441.8[医药卫生—诊断学]