人巨细胞病毒临床低传代病毒株D2的UL140基因B细胞表位生物信息学特征  

Bioinformatics characteristics of B cell epitopes of UL140 gene of human cytomegalovirus clinical low-passage virus strain D2

作　　者：苏海浩[1] 王波[1] 郭梦杰[1] 谭琪琪 胡兢晶[1] 伍苑宾[1] 晏翠芳 

机构地区：[1]广东省妇幼保健院小儿呼吸科,广州511442 [2]广州市妇女儿童医疗中心神经康复科,510180

出　　处：《中华妇幼临床医学杂志（电子版）》2017年第6期658-662,共5页Chinese Journal of Obstetrics & Gynecology and Pediatrics(Electronic Edition)

基　　金：国家自然科学基金资助项目(81070516);广东省自然科学基金资助项目(2015A030313726;2016A030313788);广东省科技计划项目(2017A020215091)~~

摘　　要：目的探讨广东省妇幼保健院分离鉴定的人巨细胞病毒(HCMV)临床低传代病毒株D2(以下简称为HCMV D2)的UL140基因B细胞表位的生物信息学特征。方法采用2016年3月至2017年3月,于广东省妇幼保健院新生儿科确诊感染HCMV的10例新生儿新鲜尿液标本中,分离鉴定的HCMV D2为研究材料。采用生物信息学方法,包括隐马尔可夫模型(TMHMM)预测HCMV D2 UL140基因跨膜结构,ExPASy在线序列分析平台预测基因的亲水性、表面可及性、极性、柔韧性及抗原性参数,以及GOR(Garnier-Osguthorpe-Robson)、人工神经网络预测法(HNN)、改进型自我优化预测结构(SOPMA)预测UL140基因二级结构。综合分析上述预测结果,并计算UL140基因的氨基酸残基抗原指数(AI),总结UL140基因B细胞表位的生物信息学特征。结果来自广东省妇幼保健院感染HCMV新生儿的HCMV D2 UL140基因的氨基酸包含191个氨基酸残基,相对分子质量为21.5×103,等电点为5.12。UL140基因B细胞表位的生物信息学特征包括:(1)TMHMM预测UL140基因存在1个跨膜区域,为26-48aa,细胞外区域为1-25aa。(2)ExPASy在线序列分析平台预测UL140基因的亲水性位于14-22、66-72、76-95、113-125、136-148、55-160、167-176aa;表面可及性位于5-22、50-60、64-96、132-160、167-187aa;极性位于5-22、45-98、103-128、132-187aa;柔韧性位于16-26、75-100、104-113、136-160aa;抗原性位于9-18、53-58、82-88、115-124、136-146、164-187aa。(3)UL140基因二级结构预测结果为,无规则卷及β-转角主要位于1-8、13-25、79-95、103-113、153-159aa。(4)计算UL140基因氨基酸残基的AI较高区域的结果显示,5-10、14-22aa区域的AI较高。结论 HCMV D2 UL140基因B细胞表位,可能位于的区域为5-10、14-22aa或其附近。Objective To investigate the bioinformatics characteristics of B cell epitopes of UL140 gene of human cytomegalovirus（HCMV） clinical low-passage virus strain D2 （to call for short as HCMV D2） isolated from infected neonates in Guangdong Provincial Maternal and Child Health Institute .Methods The HCMV D2 isolated from 10 fresh urine samples of 10 neonates with HCMV positive from Neonatology department in Guangdong Provincial Maternal and Child Health Institute were selected as research materials . Applied bioinformatics methods , including analyzed the gene transmembrane structure by Hidden Markov Model （TMHMM ）;predicted gene hydrophilicity , surface accessibility , polarity , flexibility and antigenicity of UL140 gene through ExPASy online sequence analysis platform ;the Garnier-Osguthorpe-Robson （GOR ） , hierarchical neutral network （HNN） and sawmill owners and plywood manufacturers association （SOPMA） were used to predict the secondary structure of UL140 gene .Aggregate analyzed the results above ,and calculated the antigen index （AI ） of the amino acid residues translated by UL140 gene , then summarized bioinformatics of B cell epitopes of UL140 gene .Results HCMV D2 UL140 gene contains 191 amino acid residues ,the relative molecular mass was 21 .5 × 103 and the isoelectric point was 5 .12 .The bioinformatics characteristics of B cell epitopes of UL140 gene were as follows .①TMHMM predicted UL140 gene has a transmembrane protein amino acid sequence ,and the 26-48 amino acid residues was a transmembrane region , 1-25 amino acid residues was extracellular region . ② ExPASy online sequence analysis platform for paramete prediction of UL140 gene showed that the hydrophilicity was located at 14-22 ,66-72 ,76-95 ,113-125 ,136-148 ,155-160 ,167-176 amino acid regions ;the surface accessibility was located at 5-22 ,50-60 ,64-96 ,132-160 ,167-187 amino acid regions ;the polarity was located at 5-22 ,45-98 ,103-128 ,132-187 amino acid regions ;the flexibility was located at 16-26 , 75-1

关 键 词：巨细胞病毒 临床病毒株 UL140基因 B细胞表位 婴儿 新生 

分 类 号：R722.1[医药卫生—儿科]