建立BOLD及MRS多模态fMRI针刺脑效应试验方案及其可行性研究  

Experiment design and feasibility of BOLD and MRS multimodal fMRI in analysis of brain effect induced by acupuncture

作　　者：陈媛媛[1] 赵彦萍[1] 方继良[1] 钱天翼 洪洋[1] 柳桂勇 张国雷 王珺[3] 王寅[3] 刘勇[4] 徐凯彬 李小娇[1] 

机构地区：[1]中国中医科学院广安门医院放射科,北京100053 [2]西门子医疗系统有限公司东北亚MR协调部,北京100102 [3]中国中医科学院广安门医院针灸科,北京100053 [4]中国科学院自动化研究所脑网络组研究中心,北京100190

出　　处：《中国医学影像技术》2018年第1期20-24,共5页Chinese Journal of Medical Imaging Technology

基　　金：国家自然科学基金面上项目(81273674、81774433).

摘　　要：目的建立BOLD及MRS多模态fMRI试验方案,评估针刺合谷穴后前额叶内侧皮层的负激活效应与γ-氨基丁酸(GABA)变化的关系,并优化试验流程。方法对20名健康受试者进行针刺右侧合谷穴,并行BOLD及MRS多模态fMRI扫描。MRS采用MEGA-PRESS序列,ROI为双侧前额叶内侧皮层,任务态为BOLD序列采用Block设计,设计方案为3次30s穴位刺激,间隔2min。在BOLD前后均行MRS扫描。处理图像后计算获得BOLD正负激活定量值(P_m)和ROI的GABA的定量值。结果 20名受试者均完成BOLD扫描,符合后处理要求,9名受试者MRS曲线稳定,纳入研究。20名受试者中,9名受试者呈正激活,P_m平均定量值为1.17±0.16;11名受试者呈负激活,P_m平均定量值为-1.31±0.17。9名受试者针刺前GABA平均浓度为(19.93±1.04)nmol/L,针刺后平均浓度为(20.04±0.81)nmol/L,针刺前、后GABA差值平均为(0.11±1.60)nmol/L。结论本研究建立的多模态(BOLD-fMRI及MRS)的脑功能定量研究方法成功率较高,检测到针刺时ROI的多模态脑功能变化,并可计算获得定量结果,在针刺脑效应机制研究中具有可行性。Objective To establish the methodology of combining BOLD and 1H MRS for investigating correlation between the deactivation in medial prefrontal cortex （MPFC） and gamma-aminobutyric acid （GABA） concentration by acupuncture at LI4 （Point Hegu）, and to optimize the experimental technique and procedure. Methods Twenty healthy adult volunteers were enrolled. During fMRI-BOLD scanning, each subject received acupuncture at right LI4 （Point Hegu）. MRS scanning was based on MEGA-PRESS sequence, and ROIs were located at bilateral MPFC. The task BOLD fMRI was block design, including 3 stimulations （30 s） with 2 intervals （2 min）. Then MRS scanning was performed before and after BOLD. The quantitative values of the BOLD positive and negative activations （Pro） and GABA concentrations were calculated. Results All 20 subjects completed BOLD fMRI scanning, and met the postprocessing requirements. MRS images of 9 subjects with good image quality were included in analysis. Among all 20 subjects, positive activation （Pro= 1.17± 0.16） was observed in 9, while negative activation （Pm =- 1. 31± 0. 17） was observed in 11 subjects. The GABA average values before and after the acupuncture were （19.93 ± 1.04）nmol/L and （20.04 ± 0.81）nmo1/L, respectively, and the average amplitude between post and pre acupuncture was （0. 11 ± 1.60） nmol/L. Conclusion The success rate of this method for quantitative study of brain function established multimodal-funetional （BOLD-fMRI and MRS） was acceptable, and the multimodal brain function changes as well as the quantitative values were observed in the brain region during acupuncture. Combined BOLD and MRS quantitative method is feasible for testing acupuncture response in the brain.

关 键 词：针刺 磁共振成像 血氧水平依赖 磁共振波谱 γ-氨基丁酸 

分 类 号：R3[医药卫生—基础医学] R445.2