树脂对楸灵素吸附与洗脱特性的影响  

Effect of Resins on Characteristics of Adsorption and Desorption of Catalitaxol

作　　者：冯婷婷 苗辰飞 于湘莉[1] 苗志奇[1] 

机构地区：[1]上海交通大学农业与生物学院,上海200240

出　　处：《吉林大学学报（理学版）》2018年第1期178-183,共6页Journal of Jilin University:Science Edition

基　　金：国家自然科学基金(批准号:30700061);国家重点基础研究发展计划973项目基金(批准号:2015CB755700);上海科技支撑项目(批准号:144319057001)

摘　　要：以含有楸灵素的楸树内生真菌FSN002发酵液为原料,采用静态实验法考察树脂对楸灵素的吸附过程,建立树脂吸附动力学模型,并通过模型分析定量研究各种树脂对楸灵素吸附与洗脱特性的影响.结果表明,树脂对楸灵素的吸附过程符合一级动力学模型,其中反相树脂对楸灵素的最大吸附能力平均为39.39mg/mL,是离子交换树脂的1.38倍;反相树脂吸附的楸灵素不易洗脱,平均洗脱收率为12.2%,离子交换树脂的平均洗脱收率为46.6%,其中阴离子交换树脂D201的楸灵素洗脱收率最高达83.0%,吸附速率较快,综合性能较好.Using the fermentation broth of the endophytic fungus FSN002 as material, we studied the adsorption process of resins in static test, and established dynamic model of adsorption of resins. The effect of varieties of resins on characteristics of adsorption and desorption were investigated through the model analysis. The results show that the adsorption of resins to catalitaxol fits first-order dynamic model, and the average maximum adsorption capacity of reversed-phase resins is 39.39 mg/mL, 1.38 times of ion-exchange resins. However, it is difficult to elute the catalitaxol after being adsorbed by the reversed-phase resins, and the average elution rate is 12.2%. While the average elution rate of ion exchange resins is 46.6%, and the elution rate of catalitaxol under the anion-exchange resin D201 is the highest, which can reach 83.0%. The adsorption rate is faster, and the comprehensive performance is the best.

关 键 词：大孔树脂 楸灵素 吸附 洗脱 

分 类 号：Q503[生物学—生物化学]