和胃降逆方对非糜烂性胃食管反流病大鼠5-HT3／5-HT4受体表达的影响(二)  

Researches of Hewei Jiangni Formula(和胃降逆方,HWJNF) on 5-HT3R and 5-HT4R in Rats with Non-Erosive Gastroesophageal Reflux Disease(Ⅱ)

作　　者：郭一[1] 李军祥[1] 毛堂友 陈晨[1] 谢添弘 韩亚飞[1] 谭祥[1] 孙中美 李晓红[1] 

机构地区：[1]北京中医药大学东方医院,北京100078

出　　处：《中医药导报》2018年第1期18-21,共4页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：北京中医药大学自主选题中青年教师项目(2015-JYB-JSMS117)

摘　　要：目的:观察和胃降逆方对非糜烂性胃食管反流病大鼠5-羟色胺受体表达的影响。方法:将SPF级SD大鼠随机分为空白组、模型组、奥美拉唑组、和胃降逆方高剂量组、和胃降逆方中剂量组、和胃降逆方低剂量组,采用基础致敏联合酸灌注的方法建立内脏高敏感NERD模型;干预2周后取食管组织;Western Blot与RTPCR法测定5-HT3R与5-HT4R的蛋白与基因表达水平。结果:奥美拉唑组、和胃降逆方高、中、低剂量组大鼠食管5-HT3R蛋白表达量均低于模型组(P<0.05);奥美拉唑组、和胃降逆方高、中、低剂量组大鼠食管5-HT4R蛋白表达量与模型组比较,差异均无统计学意义(P>0.05);和胃降逆方中、低剂量组大鼠食管5-HT3RmRNA表达量明显低于模型组(P<0.01或P<0.05);奥美拉唑组、和胃降逆方高、中、低剂量组食管5-HT4RmRNA表达量与模型组比较,差异均无统计学意义(P>0.05)。结论:和胃降逆方可降低非糜烂性胃食管反流病大鼠5-HT3R的表达量,调节食管-内脏高敏感性。Objective： To observe the effects of HWJNF on receptors of 5-hydroxytryptamine in rats with non-erosive gastroesophageal reflux disease. Methods： The SD rats were randomly divided into control gruup, model group, omeprazole group and the high, medium and low HWJNF groups. Except for the control group, the rats of all the other groups received basalovalbumin-sensitization combined with intra-esophageal mucosal acid exposure to establish NERD models. Then, the treatment was given for 2 weeks.The comparative observation was carried out on the expression of 5-HT3R and 5-HT4R with the methods of Western Blot and RT-PCR. Results： The omeprazole gruup and the high, medium and low ftWJNF groups showed lower expressive levels of 5-HT3R in the esophageal than the model group （P〈0.05）. However, the levels of 5-HT4R didn＇t show statistical differences among the omeprazole group and the high, medium and low HWJNF groups and model groups （P〉0.05）; The middle and low HWJNF groups showed lower expressive levels of 5-HT3RmRNA in Ihe esophageal than the model group （P〈0.01 or P〈0.05）. However, there was no difference among the omeprazole group and the high, medium and low HWJNF groups and model groups in 5-HT4RmRNA （P〉0.05）. Conclusion： HWJNF may regulate esophagus-visceral sensitivity to treat NERD according reducing 5-HT3R expression in the esophageal.

关 键 词：非糜烂性胃食管反流病 和胃降逆方 五羟色胺受体 大鼠 

分 类 号：R285.5[医药卫生—中药学]