HPLC法测定肾八味胶囊中黄酮类化合物含量  

Determination the Flavonoids Compound Content of Shenbawei Capsule(肾八味)

作　　者：唐跃年[1] 杨宇[1] 林志燕[1] 田怀平[1] 孙朝荣[1] 

机构地区：[1]上海交通大学医学院附属新华医院,上海200092

出　　处：《中医药导报》2018年第1期31-33,共3页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：上海市卫生局中医药科研基金(编号:2012G003A)

摘　　要：目的:建立测定肾八味胶囊中黄酮类化合物含量的方法。方法:采用HPLC法,样品用双蒸馏水分散后,超声提取、离心,用Inertsil ODS-3色谱柱(250 mm×4.6 mm,5μm)分离化合物,流动相为乙腈与水,梯度洗脱,流量为1 mL·min-1,柱温为室温,检测波长为215 nm,外标法定量。结果:肾八味胶囊用加样回收法与对照品比较,检出黄酮类化合物5个,芦丁、野黄芩苷、金丝桃苷、黄芩素、汉黄芩素;样品中多数峰可以达到良好分离;5个测定化合物在各自的浓度范围内均有良好的线性(r>0.999),方法回收率分别为芦丁(101.31±2.14)%、野黄芩苷(102.19±1.5)%、金丝桃苷(100.48±2.97)%、黄芩素(100.47±2.78)%、汉黄芩素(100.75±2.54)%。结论:本方法可用于肾八味胶囊中黄酮类化合物含量测定。Objective： To develop a HPLC-UV method for determination the flavonoids content of Shenbawei capsule. Methods： Samples were dissolved in water and were used ultrasonic extraction, centrifugal. The separation was performed on Inertsil ODS-3 column （250 mm×4.6mm,5μm） column with mobile phase consisted of Acetonitrile and water （gradient elution）, flow rate was 1.0 mL·min-1, at room temperature and detected with UV detector at 215 nm. External standard method was quantified. Results： As compared with standard sample, Rutin, Scutellarin, Hyperoside, Baicalein, Wogonin were checked out. Most peaks were well separated. The five compoundshowed a good linearity within the range of the respective concentrations （r〉0.999）. The method recoveries are Rutin （101.31±2.14）%, Scutellarin （102.19±1.5）%, Hyperoside （100.48±2.97）%, Baicalein （100.47±2.78）%, Wogonin （100.75±2.54）% respectively. Conclusion： The method can be used to determine the flavonoids content of Shenbawei capsule.

关 键 词：肾八味胶囊 黄酮类化合物 含量测定 

分 类 号：R284.1[医药卫生—中药学]