云南省2011-2014年急性弛缓性麻痹病例非脊髓灰质炎肠道病毒分子流行病学特征  

Molecular epidemiology of non-polio enterovirus among acute flaccid paralysis cases in Yunnan province during 2011-2014

作　　者：汤晶晶[1] 张杰[1] 李凯[1] 樊帆[1] 李琼芬[1] 丁峥嵘[1] 

机构地区：[1]云南省疾病预防控制中心,云南省肠道病毒研究中心,云南昆明650022

出　　处：《中国疫苗和免疫》2017年第6期608-612,646,共6页Chinese Journal of Vaccines and Immunization

基　　金：国家自然科学基金云南省肠道病毒分子生物学特征及监测研究(编号:81160198)

摘　　要：目的了解非脊髓灰质炎(脊灰)肠道病毒(Non-polio enterovirus,NPEV)的型别分布及分子进化特征。方法对2011-2014年从云南省急性弛缓性麻痹(Acute flaccid paralysis,AFP)病例中分离到的148株NPEV进行VP1完整编码区核苷酸的扩增与序列测定。所获得的云南地方株基因序列与各基因型原型株进行核苷酸与氨基酸同源性比较,并与Gen Bank中选取的代表株构建系统进化树。结果在148株NPEV分离株中,肠道病毒(Enterovirus,EV)A组(EV-A)、EV-B、EV-C分别占31.1%(46株,8个血清型)、64.9%(96株,27个血清型)、4.1%(6株,3个血清型);基因进化树显示,各种血清型病毒株与对应原型株及代表株聚集;除埃可病毒10型(E-10)和EV-C96型外,云南地方株与原型株位于不同分支。在4年的流行过程中,同一个型别的分离株之间的遗传变异程度最高的是EV-C96、E-18和E-5,分别达到21.5%、21.3%和20.4%。结论云南省存在EV多个型别的循环,需持续性监测和探索EV分子流行病学和进化规律。Objective To determine the genotype distribution and molecular evolution of non-polio enterovirus（ NPEV） in Yunnan province. Methods We sequenced and analyzed the entire VP1 coding regions of 148 NPEV strains isolated from acute flaccid paralysis（ AFP） cases in Yunnan from 2011 to2014. We compared the homologies of nucleotide and amino acid sequences between Yunnan stains and prototype strains,and we constructed and compared phylogenetic trees of nucleotide sequences between Yunnan stains and other reference strains from Gen Bank. Results Among 148 isolated NPEV stains,enterovirus A（ EV-A）,EV-B,and EV-C accounted for 31. 1%（ 46 strains,8 serotypes）,64. 9%（ 96 strains,27 serotypes）,and 4. 1%（ 6 strains,3 serotypes）,respectively. Phylogenetic analysis showed that each Yunnan strain serotype clustered with its prototype strain or a representative strains;Yunnan strains,except for echovirus 10（ E-10） and EV-C96,and prototype strains were located in different phylogenetic branches. During 4 years of circulation,the strains with the most genetic mutations among the same serotypes were EV-C96,E-18 and E-5（ 21. 5%,21. 3% and 20. 4%,respectively）.Conclusions Multiple serotypes of EVs co-circulated in Yunnan. Continuous surveillance should be maintained to explore the molecular epidemiology and evolution of EVs.

关 键 词：急性弛缓性麻痹 非脊髓灰质炎肠道病毒 分子流行病学 

分 类 号：R373.2[医药卫生—病原生物学]