激发态分子内质子转移荧光探针的研究进展  

Advanced in Fluorescent Probes Based on Excited State Intramolecular Proton Transfer

作　　者：王德佳 徐勇前 孙世国 李红娟 

机构地区：[1]西北农林科技大学化学与药学院,陕西省天然产物与化学生物学重点实验室,陕西杨凌712100

出　　处：《应用化学》2018年第1期1-20,共20页Chinese Journal of Applied Chemistry

基　　金：国家自然科学基金资助项目(21676218,21476185,21472016,21272030)

摘　　要：荧光探针凭借其选择性好、灵敏度高、响应时间快、易于操作和检测限低等优点得到了广泛的关注。激发态分子内质子转移(ESIPT)化合物具有特殊的激发态光物理过程,其显著的光物理性质是有较高的荧光量子产率及大的斯托克斯位移。对于荧光分子而言,较大的斯托克斯位移可以减少自吸收和由内滤效应产生的干扰,增强分子的耐光性,有利于荧光的发射。本文对ESIPT荧光探针检测离子(包括金属阳离子和阴离子)、中性小分子和生物大分子的研究进展进行阐述,并对ESIPT荧光分子的存在问题和应用前景进行评述。Fluorescent probes are gaining more and more attention because of their high selectivity, sensitivity, fast response, simple operation and low detection limit. Compounds based on excited state intramolecular proton transfer（ESIPT） have special excited state photophysical properties, such as high fluorescence quantum yield and large Stokes shift. For fluorescent molecules, the larger Stokes shift can reduce the interference caused by self-absorption and internal filtration, and enhance light-fastness of the molecules and facilitate the emitting of fluorescence. In this review, we summarized these ESIPT fluorescent probes from the classification of targets. The targets for detection were divided into ions（including metal cations and anions）, neutral small molecules and biological macromolecules. The existing problems and application prospects of fluorescent molecules based on ESIPT were commented.

关 键 词：激发态分子内质子转移 金属阳离子 阴离子 中性小分子 生物大分子 荧光探针 

分 类 号：O652[理学—分析化学]