四川紫花白及无菌播种技术研究  

Research on the Aseptic Seeding Technique of Bletilla striata with Lilac Flowers

作　　者：姜丽琼[1] 肖欣怡 李文俊[1] 肖前刚[1] 徐志萍[1] 廖兴勇[1] 陈文灵[1] 李路[1] 

机构地区：[1]成都市农林科学院,四川成都611130 [2]成都市实验外国语学校,四川成都610031

出　　处：《四川林业科技》2017年第6期51-54,共4页Journal of Sichuan Forestry Science and Technology

基　　金：成都市2016年产业集群协同创新重大项目<成都林竹新品种及高效丰产技术研究与示范>支持

摘　　要：以当年成熟白及果荚种子作为外植体,开展组织培养试验研究。结果表明:最佳外植体诱导培养基为改良MS+0.2 mg·L^(-1)6-BA+0.2 mg·L^(-1)NAA,发芽率可达90%~100%;最佳增殖培养基为改良MS+2.0 mg·L^(-1)6-BA+0.3 mg·L^(-1)NAA,增殖系数3.4;最佳生根培养基为改良MS+0.2 mg·L^(-1)NAA+1 mg·L^(-1)AC+50 g·L^(-1)香蕉泥培养基,生根率为95.6%。炼苗基质为腐殖土:珍珠岩:椰糠:河沙=5:1:2:2组合,为白芨移栽最佳基质,移栽苗存活率可达97%。Abstract：Taking the ripe seeds that removed from the inflated pods as explant, experiments were conduc- ted on this tissue culture. The results showed that the best inductive medium for explant culture was an improved fromula listed as follows ： MS ＋ 0. 2 mg · L- 16 - BA ＋ 0. 2 mg · L- 1NAA, and the germination rate could be generally up to 90% ~ 100% ;The best enriched medium was an improved formula listed as follows ：MS ＋2. 0 mg · L-6 - BA ＋0. 3 mg · L-1NAA,and the propagation coefficient was 3.4;The best rooting medium was an improved formula of banana puree substrate listed as follows ： MS ＋ 0. 2 mg· L- 1 NAA ＋ 1 mg.L-IAC ＋50 g· L-1 ,and the rate of rooting was 95.6%. The growth substrate was a combi- nation of humus：perlite： coconut hust： fluvial sand = 5： 1：2： 2, which was the best transplanting substrate for Bletilla striata, and the survival rate of the transplanting reached 97 %.

关 键 词：紫花白及 无菌播种 

分 类 号：S567.239[农业科学—中草药栽培]