一株新型嗜水气单胞菌拮抗菌的筛选及鉴定  

Screening and Identification of a New Antagonistic Bacteria Against Aeromonas hydrophila

作　　者：张皎皎 马富平 熊波[1,2,3] 苏胜齐[1,2,3] 吴正理 姚维志[1,2,3] 

机构地区：[1]西南大学动物科技学院,重庆400715 [2]西南大学渔业资源环境研究中心,重庆400715 [3]淡水鱼类资源与生殖发育教育部重点实验室,重庆400715

出　　处：《西南大学学报（自然科学版）》2017年第12期18-23,共6页Journal of Southwest University(Natural Science Edition)

基　　金：全国优秀博士学位论文作者专项基金(201473);中央高校基本科研业务费专项资金项目(XDJK2015A001;SWU114011;XDJK2015C012;XDJK2017D036)

摘　　要：从草鱼养殖池塘的水样及底泥样品中筛选对嗜水气单胞菌有拮抗作用的菌株,初步研究其抑菌效果,为水产养殖过程中嗜水气单胞菌的生物防治提供菌株资源.以嗜水气单胞菌为指示菌,采用点喷法进行拮抗性菌株初筛,采用牛津杯法进行复筛;通过细菌形态观察、生理生化特性试验、16SrDNA基因测序和系统发育分析,对筛选菌株进行分类鉴定.得到1株对嗜水气单胞菌有明显抑制作用的拮抗菌株WM-1,抑菌圈直径达21.8mm,经鉴定为甲基营养型芽孢杆菌(Bacillus methylotrophicus).该菌筛选得到的拮抗菌WM-1,对嗜水气单胞菌抑制效果显著,为进一步研究其抑菌机理提供了菌种资源.The objective of this study was to screen and characterize an antagonistic strain against Aeromonas hydrophilafrom the water and sediment samples of a grass carp culture pond.In the present study,we use A.hydrophilaas the index bacteria,use the point spraying method and the Oxford cup method for the antagonistic bacteria primary screening and second screening,respectively.The isolated antagonistic bacteria were identified by morphological characteristics,physiological and biochemical characteristics,16 SrDNA sequence and phylogenetic analysis.From the water and sediment samples of the grass carp culture pond,an antagonistic strain WM-1 against A.hydrophila was selected,whose antibacterial circle diameter to the pathogens was 21.8 mm.Strain WM-1 was identified as Bacillus methylotrophicus.A strain（WM-1）with high antagonistic activity against A.hydrophila has been selected.This research provides a new microorganism resource to further study the antibacterial mechanism against A.hydrophila.

关 键 词：拮抗菌 嗜水气单胞菌 甲基营养型芽孢杆菌 

分 类 号：S941[农业科学—水产养殖]