水热法一步合成氮掺杂碳点及其在Cu(Ⅱ)离子检测中的应用  

One-Step Hydrothermal Synthesis of Nitrogen Doped Carbon Dots and Their Application in Cu(Ⅱ) Ion Detection

作　　者：周希 刘美红[1] 王春鹏[1,2] 储富祥[1,2] 

机构地区：[1]中国林业科学研究院林产化学工业研究所,南京210042 [2]中国林业科学研究院林业新技术研究所,北京100091

出　　处：《化学通报》2018年第1期65-69,共5页Chemistry

基　　金：中央级公益性科研院所基本科研业务费专项(CAFYBB2017ZX003);中国林科院林业新技术研究所基本科研业务费专项(CAFYBB2017SY033)资助

摘　　要：采用水热法以聚乙烯亚胺为原料一步制备氮掺杂荧光碳量子点。紫外-可见吸收光谱、荧光光谱以及透射电镜显示,所制备的碳点荧光性能优异、分散性好、且无团聚现象。在0.1mol/L PBS溶液中,荧光碳点的荧光强度随着Cu(Ⅱ)浓度的增加逐渐减弱。该方法对Cu(Ⅱ)检测的线性范围为50~150μmol/L,检出限为10μmol/L。细胞毒性测试结果表明,不同浓度的碳点对细胞活性影响均较小,其细胞毒性低。以上结果说明该碳点能成功检测Cu(Ⅱ)且细胞毒性低,在生物传感方面有潜在应用价值。The nitrogen doped carbon dots based on polyethyleneimine were prepared by one-step hydrothermal method. Ultraviolet-visible absorption, fluorescence spectra and transmission electron microscopy show that the carbon dots have excellent fluorescence performance and good dispersion. In 0. 1 mol/L PBS solution, the fluorescence intensity of carbon dots reduces as Cu（ Ⅱ ） concentration increases gradually. The detection linear range of Cu（ Ⅱ ） is 50 - 150 μmol/L with the detection limit of 10 μmol/L. Cytotoxicity test results show that different concentrations of the carbon dots all have low toxicity. The above results show that the carbon clots can successfully detect Cu（ Ⅱ ） with low cytotoxicity, showing potential application in biological sensing. Keywords Carbon materials, Fluorescence, Quenching, Cell toxicity

关 键 词：碳材料 荧光 猝灭 细胞毒性 

分 类 号：O657.3[理学—分析化学]