甘蓝型油菜矮化突变体的茎秆发育转录组分析  

Transcriptome analysis on stem development of a Brassica napus L. dwarf mutant

作　　者：牛显飞 蒋学飞 解艳芳 刘双双 王茂林 

机构地区：[1]四川大学生命科学学院生物资源与生态环境教育部重点实验室,成都610065

出　　处：《四川大学学报（自然科学版）》2018年第1期201-206,共6页Journal of Sichuan University(Natural Science Edition)

基　　金：国家重点研发计划(2016YFD010020002);国家科技支撑计划(2013BAD01B03);"十三五"四川省作物育种攻关项目(16ZC2860)

摘　　要：甘蓝型油菜矮化突变体"NDF-1"是从高秆油菜"3529"理化诱变群体中分离的矮化突变体,它的株高只有高秆亲本的1/3,是一个优良的甘蓝型油菜矮化资源.本文应用RNA-Seq测序技术分析了"NDF-1"茎开始伸长的抽薹期和花期转录组,为深入了解油菜茎秆发育机理提供基因组层面的参考.抽薹期和花期的测序数据通过筛选得到了4.55亿条质量合格的clean reads.经过对高质量序列的组装注释,筛选得到"NDF-1"和"3529"间的2147条差异表达基因(DEGs).选择其中的20个差异表达基因进行了荧光定量PCR验证.通过GO和KEGG分析,发现"NDF-1"的矮化与赤霉素、生长素和油菜素内酯相关,细胞伸长异常和细胞壁的不正常形成也影响"NDF-1"的茎秆发育.从差异基因中筛选出与植物矮化性状相关的基因,为利用矮化基因改善性状和矮化机理的研究和提供了更多信息.＂NDF-I＂ is a Brassica napus L. dwarf mutant, which was derived from physical and chemical mutagenesis of a high stemmed line ＂3529＂, possesses a stable dwarf phenotype of approximately one third the height of high stalk parent ＂3529＂. It is an excellent resource dwarf Brassica napus L. RNA- Seq based transcriptome analysis on stem elongation of the dwarf mutant from Bolting Stage （BS） and Florescence Stage （FS）, which was conducted to provide a reference for the genome level in order to un- derstand the mechanism of rapeseed stem development. A total of 455 million clean reads were obtained from two different stages include Bolting Stage （BS） and Florescence Stage （FS）. Then 2,147 differen- tially expressed genes （DEGs） were found which detected between ＂NDF-I＂ and ＂3529＂ after assembled them. Furthermore, twenty differentially expressed genes were selected for RT-PCR validation. With the GO and KEGG pathway analysis, ＂NDF-I＂ stems elongation most regulated by the gibherellin,auxin and brassinosteroids, cell wall formation or cells Elongation is not normal but also play an important role in ＂NDF-I＂ dwarf. Screening genes associated with plant dwarfing traits from DEGs, which provide a reference for further use of these genes on traits improvement and dwarfing mechanism research.

关 键 词：甘蓝型油菜 矮化 茎秆伸长 转录组 

分 类 号：Q811.4[生物学—生物工程]