贵州省百日咳频发地区605份健康人血清百日咳抗体水平检测  

Pertussis antibody level in serum among the 605 healthy population from high-prevalence area,Guizhou

作　　者：张德著[1] 刘淳婷[1] 万永虎[1] 蒋凤[1] 刘铭[1] 刘清 管庆虎[1] 王涛[1] 李祝薇 叶新贵[1] 

机构地区：[1]贵州省疾病预防控制中心,贵州贵阳550004 [2]钟山区疾病预防控制中心,贵州六盘水553000 [3]威宁县疾病预防控制中心,贵州毕节551700

出　　处：《现代预防医学》2018年第1期164-168,共5页Modern Preventive Medicine

基　　金：贵州省疾病预防控制中心青年基金(No:2016-E-7青)

摘　　要：目的了解贵州省百日咳病例频发地区健康人群抗体水平,评估真实发病水平和疾病风险,为其预防和控制提供依据。方法 2016年12月在贵州省钟山区和威宁县2个百日咳频发地区,采用分层抽样和随机抽样的方法采集健康人群的静脉血清3~5ml;通过酶联免疫吸附实验(ELISA)定量测定血清中百日咳鲍特菌总IgG含量;采用Microsoft Excel软件整理测定结果,通过SPSS 18.0,选用方差分析和卡方检验等统计方法进行数据分析。结果共检测钟山区和威宁县健康人群血清605份,百日咳鲍特菌总IgG阳性率为10.41%,其浓度几何均值为10.46IU/ml;男女性别间百日咳鲍特菌总IgG抗体阳性率(10.55%,10.00%)和抗体浓度(9.91IU/ml,10.95IU/ml)均无统计学差异(=0.049,P=0.893;F=2.957,P=0.355);4剂次无细胞百白破疫苗(DTa P)免疫人群与3剂次DTa P疫苗免疫人群间的百日咳鲍特菌总IgG浓度分别为2.96IU/ml、2.76IU/ml,二者间百日咳鲍特菌总IgG浓度差异无统计学意义(F=6.757,P=0.694);0-3岁人群百日咳鲍特菌总IgG浓度随接种时间推移而下降,末次接种疫苗后5个月内为3.61IU/ml,2-3年后降至2.19IU/ml(F=2.731,P=0.035);受百日咳感染影响,青少年和成人百日咳鲍特菌总IgG浓度有较明显的峰值,抗体水平随年龄增长呈波动性上升。结论贵州省百日咳频发的钟山区和威宁县健康人群血清中百日咳鲍特菌总IgG水平较低,可能存在接受疫苗免疫的人群仍然感染百日咳的现象,需进一步加强监测,优化免疫策略。Objective To survey the level of Pertussis antibody level among the healthy population from the area with a high frequent report rate of Pertussis in Guizhou province, to evaluate the true level and risk factors of the disease, and to provide evidence for disease prevention and control. Methods stratified sampling and random sampling method were used to sample healthy person lived ZhongShan district and WeiNing country. 3 - 5 milliliters serum of venous blood was collected from each sampled person. Pertussis immunoglobulin G （IgG） antibody was measured by enzyme - linked immunosorbent assay （ ELISA ） ~ Microsoft Excel was used to organize the result of test, and analysis of variance and chi - square test were conducted with SPSS18. 0. Results 605 serum samples collected from the two high prevalence areas were tested,the IgG antibody positive rate was 10. 41% and the geometric mean of concentration was 10. 46IU/ml. In male and female, the IgG antibody positive rates were 10. 55% and 10.00% and the average concentration was 9.91IU/ml and 10. 95IU/ml in male and female respectively, both of difference was not statistically significant （ x2 = 0. 049, P = 0. 893 ; F = 2. 957, P = 0. 355 ）. There was no significant difference （ F = 6. 757, P = 0. 694 ） between the population inoculated 3 doses and 4 doses of the Diphtheria, Tetanus Toxoids and acellular Pertussis Vaccine （DTaP） , the concentration of IgG was 2. 96IU/ml and 2. 76IU/ml respectively, the concentration of IgG was decreasing with inoculation time among the people under 3 years old, from 3. 61 IU/ml within 5 months after inoculation to 2. 19IU/ml after 2 -3 years（F =2. 731 ,P =0. 035）. Due to infection influence,there were two slightly peaks of IgG among teenagers and adults, antibody concentration presented a fluctuated increasing trend with age growth. Conclusion Currently, the pertussis antibody level of serum among the healthy population in ZhongShan district and WeiNing country of Guizhou Province is relatively low, although vacc

关 键 词：健康人群 百日咳 抗体 

分 类 号：R195[医药卫生—卫生统计学]