全基因组水平上芸薹属Mlo抗病基因的比较分析  

Genome-wide comparative analysis of MLO related genes in Brassica lineage

作　　者：闫沛喆 周索[1] 李雪辉 赵姗姗 周欢欢 周业皓 徐树林[1] 柯涛[1] 

机构地区：[1]南阳师范学院,河南南阳473061

出　　处：《中国油料作物学报》2017年第6期729-736,共8页Chinese Journal of Oil Crop Sciences

基　　金：河南省科技厅产学研合作项目(162107000007)

摘　　要：为揭示植物广谱抗病机制,创造优良的抗白粉病新抗源,了解芸薹属全基因组水平的MLO基因家族,利用保守结构域的隐马模型,分析拟南芥、白菜、甘蓝和油菜中123个MLO类型抗病基因。进化分析发现,拟南芥和芸薹属物种Mlo抗病基因的进化树非常清晰地分成3个亚组。关系分析显示,拟南芥Mlo3和Mlo9基因在芸薹属物种中并没有找到对应的同源基因。通过跨膜结构域分析发现,71个MLO类型抗病基因有超过7个跨膜结构域,且包含超过12个外显子结构。这些基因符合MLO类型抗病基因的典型特征,可能是四个物种中主要的功能基因。通过对71个Mlo抗病基因的保守结构域分析发现,四个物种中均含有1个共有的保守氨基酸序列(长度462aa),这段保守的氨基酸序列可能是Mlo基因的主要功能序列。除了Mlo3和Mlo9,其余Mlo基因都发生了不同程度的扩张。For novel insights on MLO gene family in Brassica plants,and for revealing spectrum of plant disease resistance to powdery mildew,MLO genes family in Brassica species and Arabidopsis thaliana were studied.Conserved domain HMM profile was used. 123 Mlo resistant genes in Brassica rapa,B. oleracea and B. napus whole genomes were focused. Evolutionary analysis on MLO gene family among the 4 species showed that they were clustered into 3 different subgroups. Homologous relationship showed that Mlo3 and Mlo9 genes in A. thaliana had no homologous genes in Brassica species. Among the transmembrane motifs of 123 Mlo genes,more than 7 transmembrane motifs in 71 members were found,which contained more than 12 exons. These results were consistent with typical characteristics of Mlo and might be the major functional genes among these 4 species. Conserved domains of71 Mlos in all the species had one conserved amino acid sequence（ 462 aa long）,which might be the main functional domain. Further comparison of Mlos in A. thaliana and Brassica species uncovered that most of Mlos in A.thaliana had experienced different expansion in Brassica species except Mlo3 and Mlo9.

关 键 词：抗病基因 MLO基因家族 芸薹属 进化分析 

分 类 号：S565.4[农业科学—作物学]