小桐子转录因子JcZAT10基因的分离与低温表达分析  

Isolation and chilling expression of JcZAT10 gene from Jatropha curcas

作　　者：王海波[1,2] 龚明[3] 刘潮[1,2] 高永[1,2] 代冬琴 唐利洲[1,2] 

机构地区：[1]曲靖师范学院云南高原生物资源保护与利用研究中心,云南曲靖655011 [2]曲靖师范学院云南省高校云贵高原动植物遗传多样性及生态适应性重点实验室,云南曲靖655011 [3]云南师范大学生命科学学院,云南昆明650500

出　　处：《中国油料作物学报》2017年第6期805-812,共8页Chinese Journal of Oil Crop Sciences

基　　金：国家自然科学基金(31460179;31260064);云南省高校科技创新团队支持计划"云南省高校云南特境内生菌资源的开发与利用科技创新团队"

摘　　要：利用RT-PCR技术克隆小桐子C2H2型锌指蛋白ZAT10转录因子基因c DNA序列,命名为Jc ZAT10,并对其基因结构、功能结构域、系统进化及差异表达进行了分析。结果表明,小桐子Jc ZAT10基因开放阅读框全长753bp,编码250个氨基酸,推测蛋白分子量为26.7k Da,理论等电点为8.45,并鉴定到两个锌指结构域、核定位信号序列以及富Leu保守基序。系统进化分析显示,Jc ZAT10与同属大戟科的蓖麻亲缘关系最近,序列一致性为79.6%。qRT-PCR分析表明,小桐子Jc ZAT10基因存在组织表达特异性,在茎中表达量较高,而在叶片中表达量较低,但在叶片与根中受低温诱导表达,且都在低温胁迫3h时达到最大表达量。本研究为进一步对Jc ZAT10基因进行功能验证以及其在低温信号转导中的机制研究提供了依据。C2H2-type zinc finger protein is the key transcription factor family in plant stress resistance. A new ZAT10 gene belongs to C2H2-type zinc finger protein family was obtained from Jatropha curcas by RT-PCR,then the gene structure,functional motifs,phylogenetic relationship,and differential expression were systematically analyzed. The result showed that the full-length ORF of Jc ZAT10 was 753 bp,encoded a 250 amino acid polypeptide with the molecular weight of 26. 7 k Da and the p I value of 8. 45. Jc ZAT10 protein contained 2 zinc finger domains,nuclear localization signal sequence,and conserved Leu-rich motif. Its amino acid sequence shared79. 6% identity with Ricinus communis,which belonged to the same family of Euphorbiaceae. qRT-PCR analysis revealed that Jc ZAT10 expressed in different organs,with abundant expression in stem,but scarcely in leaves,and remarkably cold-induced expression in leaf and root,with the highest expression levels at 3 h treatment. This study laid a foundation for further studies on Jc ZAT10 function verification and its mechanism elucidation of cold signaling transduction in J. curcas.

关 键 词：小桐子 锌指蛋白 ZAT10 基因克隆 低温胁迫 表达分析 

分 类 号：S565.9[农业科学—作物学]