拳参提取物-413对心肌肥厚小鼠心肌缝隙连接蛋白Cx43的影响  

Effect of PBNA-413 on Connexin 43 in Myocardial Hypertrophy Mice

作　　者：严文华[1] 曾雪亮[1] 赵雅欣 许萍萍 曾洁[1] 曾丽梅[2] 黄志华[2] 

机构地区：[1]赣南医学院第一附属医院,江西赣州341000 [2]赣南医学院基础医学院,江西赣州341000

出　　处：《赣南医学院学报》2017年第6期856-858,864,共4页JOURNAL OF GANNAN MEDICAL UNIVERSITY

基　　金：江西省自然科学基金项目(NO.20122BAB205039);江西省卫生厅中医药科研基金课题(NO.2012A015);江西省中医药课题(NO.2009ZDS12700)

摘　　要：目的:研究拳参提取物(PBNA)-413对异丙肾上腺素(Iso)诱导心肌肥厚小鼠心肌组织缝隙连接蛋白表达的影响。方法:将小鼠随机分为正常对照组、模型组、卡托普利组(阳性对照组)、低和高剂量PBNA-413组。采用小鼠背部皮下注射Iso,连续10 d,制备心肌肥厚模型;连续给药14 d后,称小鼠体重、全心重、左心室重,计算全心重量指数、左心室重量指数;取左心室心肌组织,Western blot方法检测connexin-43(Cx43)磷酸化及总蛋白水平。结果:模型组小鼠心脏重量指数及左心室重量指数均升高,Cx43的磷酸化和总蛋白水平均降低;PBNA-413治疗后,心肌肥厚模型组小鼠心脏重量指数及左心室重量指数均降低,Cx43的磷酸化和总蛋白水平升高。结论:PBNA-413能够通过调节心肌组织缝隙连接的重构延缓心肌肥厚进程及预防心律失常并发症。Objective： To investigate the effect of Polygonum Bistorta L.n-butyl alcohol（PBNA-413） on the expression of myocardial Cx43 in the myocardium hypertrophied mice induced by isoproterenol（Iso）.Methods： The mice were randomly divided into normal control group,model group,captopril group（positive control group）,low and high dose of PBNA-413 group.The mice were injected subcutaneously with Iso on the back of mice for 10 days to prepare a model of cardiac hypertrophy.After 14 days of continuous administration,the mice were weighed with weight,heart weight and left ventricular weight.The cardiac weight index and left ventricular weight index were calculated.The phosphorylation of connexin-43（Cx43） and total protein were detected by Western blot in the left ventricular myocardium.Results： The cardiac weight index and left ventricular weight index of the model group were increased,and the phosphorylation and total protein levels of Cx43 decreased.After the treatment of PBNA-413,the cardiac weight index and left ventricular weight index in the model group were decreased,and the phosphorylation and total protein levels of Cx43 were increased.Conclusion： PBNA-413 can delay the process of cardiac hypertrophy and prevent arrhythmia complications through the regulation of myocardial tissue gap junction reconstruction.

关 键 词：拳参 心肌肥厚 心律失常 缝隙连接蛋白43 

分 类 号：R285[医药卫生—中药学]