沙白肺炎合剂对小鼠非特异性免疫和体液免疫功能调节作用实验研究  

Experimental Study of Moderating Effect of Shabai Pneumonia Mixture on Non-special Immunity and Humoral Immunity Function

作　　者：彭波[1] 王雷[2] 龙飞 李松桃 蒲蓉[1] 杨仁旭[1] 

机构地区：[1]成都中医药大学附属医院/临床医学院,四川成都610072 [2]成都中医药大学临床医学院,四川成都610075 [3]四川省彭州市中医院,四川彭州611930

出　　处：《实用中医药杂志》2018年第1期1-3,共3页Journal of Practical Traditional Chinese Medicine

基　　金：成都中医药大学科技发展基金(ZRYB200921)

摘　　要：目的:观察沙白肺炎合剂对小鼠非特异性免疫和体液免疫功能的调节作用。方法:210只小鼠分为3组各70只。将第1实验大组小鼠70只随机分为正常对照组、HC(氢化可的松)组、沙白肺炎合剂低剂量组、沙白肺炎合剂中剂量组、沙白肺炎合剂高剂量组、HC加沙白肺炎合剂组、多抗甲素组。将第2和第3实验大组小鼠各70只随机分为正常对照组、CY(环磷酰胺)组、沙白肺炎合剂低剂量组、沙白肺炎合剂中剂量组、沙白肺炎合剂高剂量组、CY加沙白肺炎合剂组、多抗甲素组。按照实验设计方案给各组小鼠用药或生理盐水10天后处死各组小鼠,提取标本后进行相应指标检测。采用巨噬细胞吞噬鸡红细胞法测定沙白肺炎合剂对小鼠腹腔巨噬细胞吞噬功能的影响,称取胸腺和脾脏湿重法测定沙白肺炎合剂对胸腺和脾指数的影响,用流式细胞仪检测靶细胞死亡率法测定沙白肺炎合剂对NK细胞活性的影响,用定量溶血分光光度测定法检测对小鼠抗体生成细胞的影响,用丰数溶血值(HC50)测定法检测对小鼠血清溶血素形成的影响。通过组间对比分析各组小鼠非特异性免疫和体液免疫功能的差异性,从而判断沙白肺炎合剂对小鼠非特异性免疫和体液免疫功能调节作用。结果:沙白肺炎合剂中、高剂量对小鼠腹腔巨噬细胞吞噬功能、胸腺和脾指数、NK细胞活性、抗体生成细胞、血清溶血素形成均具有促进作用(P<0.05),且浓度增加作用更明显(P<0.05)。结论:沙白肺炎合剂对小鼠非特异性免疫和体液免疫功能具有调节作用。Objective： To evaluate the regulation of the Shabai Pneumonia Mixture on nonspecific immumty and humoral immunity in mice. Method： A total of 210 mice were divided into three large groups, 70 mice in each group. The 70 mice in the first group were randomly divided into 7 groups： normal control group, the HC （ hydrocortisone ） group, the ShaBai Pneumonia Mixture （low-dose） group, the ShaBai Pneumonia Mixture （mid-dose） group, the ShaBai Pneumonia Mixture （ high-dose ） group, the HC ＋ ShaBai Pneumonia Mixture group and the Polyresistm group. Likewise, 70 mice in the second and the third experiments were randomly divided into 7 groups： normal control group, the CY （ cyclophosphamide ） group, the ShaBai Pneumonia Mixture （ low-dose ） group, the ShaBai Pneumonia Mixture （ mid- dose ） group, the ShaBai Pneumonia Mixture （high-dose） group, the CY ＋ ShaBai Pneumonia Mixture group and the Polyresistin group. Medicine or physiological saline solution were administered in each group according to the experimental design.10 days later, the mice were executed, the specimens were collected and the corresponding indicators were detected. Chicken red blood cells of macrophage cell was used to evaluate the effect of the ShaBai Pneumonia Mixture on abdominal macrophage phagocytosis of mice. Weighing hymus and spleen wet weight method was employed to determine the influence on thymus and spleen index by Sha Bai pneumonia mixture. The effect of ShaBai Pneumonia Mixture on INK cell activity was measured by flow cytometry. Quantitative hemolysis spectrophotometry was used to detect the effect of antibody generating cells in mice. Abundant number of hemolysis value （ HCS0 ） assay was used to detect the impact on the formation of serum hemolysin in mice. Through the comparison among groups, the differences of nonspecific immunity and humoral immunity function in every group of mice was analyzed to judge regulatory effect of the nonspecific immunity and humoral immunity function in mice thr

关 键 词：沙白肺炎合剂 小鼠 非特异性免疫 体液免疫 实验研究 

分 类 号：R289.5[医药卫生—方剂学]