应用RACE法克隆雷竹笋PAL基因及序列分析  

Cloning of PAL Gene from Bamboo Shoot(Phyllostachys praecox)by RACE Method and Its Sequence Analysis

作　　者：张规富 汪浩 

机构地区：[1]湖北科技学院核技术与化学生物学院,湖北咸宁437100

出　　处：《江西农业学报》2018年第1期1-6,共6页Acta Agriculturae Jiangxi

基　　金：湖北省自然科学基金项目(2013CFC010);湖北科技学院校内科研项目(2016-19XB01)

摘　　要：应用RACE法,克隆获得了雷竹笋PAL基因的全长序列,并对其进行了生物信息学分析。结果表明:雷竹笋PAL基因的开放阅读框长度为2103 bp,共编码701个氨基酸,其中具有1个保守的活性位点Ala-Ser-Gly(A-S-G);在PAL蛋白的二级结构中含有大量的α螺旋和β折叠,其三级结构模型为一个带柄的圆锥形。构建的PAL基因核苷酸序列进化树结果显示雷竹与毛竹、绿竹的亲缘关系最近,与小麦、大麦、水稻、佛肚竹的亲缘关系较近,与玉米、高粱的亲缘关系较远。RACE method was applied to clone and obtain the full-length sequence of PAL gene from bamboo shoot（Phyllostachys praecox）,and its bioinformatics analysis was carried out.The results indicated that：the open reading frame of the cloned PAL gene from bamboo shoot was 2103 bp in length,it encoded 701 amino acids,and had a conservative activity site Ala-SerGly（A-S-G）; there were a large number of α-helix and β-sheet in the secondary structure of PAL protein,and the tertiary structure model of PAL protein was a tapered cone with a handle.The phylogenetic tree analysis of nucleotide sequences in PAL gene showed that P.praecox had the closest affinity relationship with Phyllostachys edulis and Bambusa oldhamii,had a closer affinity relationship with Triticum aestivum,Hordeum vulgare,Oryza sativa and Bambusa ventricosa,and had a farther affinity relationship with Zea mays and Sorghum bicolor.

关 键 词：雷竹笋 RACE技术 克隆 PAL基因 序列分析 蛋白质结构 

分 类 号：S644.2[农业科学—蔬菜学]