成人脂肪细胞β3肾上腺素受体表达与肥胖的相关性  

Relationship between adipocyte β3 adrenergic receptor expression level and adiposity in adults

作　　者：曹文悦 蒲迎[1] 刘钊[3] 郭峰 于静[5] 尹晓[1] 

机构地区：[1]山东大学附属济南市中心医院内分泌科,山东济南250013 [2]山东大学齐鲁医学院,山东济南250012 [3]山东大学附属济南市中心医院肝胆外科,山东济南250013 [4]山东大学附属济南市中心医院泌尿外科,山东济南250013 [5]山东大学附属济南市中心医院手术室,山东济南250013

出　　处：《山东大学学报（医学版）》2018年第1期81-85,共5页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金(81300686)

摘　　要：目的探讨成人脂肪细胞β3肾上腺素受体(β3AR)表达水平的变化及与体质量指数(BMI)的关系,为通过干预β3AR信号通路治疗肥胖症提供理论依据。方法纳入择期腹部手术患者42例,取脂肪组织,根据BMI分为超重组(25≤BMI<30)和正常体质量组(BMI<25)。通过实时定量PCR和Western blotting技术检测成熟脂肪细胞、前脂肪细胞中β3AR的mRNA及蛋白表达水平。结果超重组成熟脂肪细胞β3AR mRNA(t=2.594,P=0.013)和蛋白(t=2.760,P=0.010)及前脂肪细胞β3AR蛋白(t=2.442,P=0.023)表达水平均低于正常体质量组。成熟脂肪细胞β3AR mRNA表达与BMI呈负相关关系(r=-0.406,P=0.008)。结论肥胖前期的超重患者脂肪细胞β3AR表达水平下调,可能参与肥胖的发生过程。Objective To detect relationship between adipocyte β3AR expression and adiposity in adults, and to explore the potential therapeutic target for obesity. Methods A total of 42 adipose tissues were collected from the patients who accepted abdominal operation and divided into overweight group （25 ≤ BMI〈30） and normal weight group （BMI〈 25）. β3AR expressions in mRNA and protein levels were detected by qRT-PCR and Western blotting. Results The 133AR mRNA of mature adipocyte （t=2.594, P=0.013）, β3AR protein level of mature adipocyte （t=2.760, P= 0.010） and preadipocyte （ t= 2.442, P= 0.023） in overweight group were lower than that in normal weight group. There was negative correlation between β3AR mRNA expression and BMI （r=-0.406, P= 0.008）. Conclusion The down- regulation of adipocyte β3AR expression in overweight group may play an important role in development of obesity.

关 键 词：Β3肾上腺素受体 脂肪细胞 超重 成人 

分 类 号：R589.2[医药卫生—内分泌]