长远航海员皮肤共生微生物宏基因组分析  

Metagenomic analysis of symbiotic microorganism in sailors with long sea voyage

作　　者：郑巍薇[1] 代淑阳 乔媛媛[1] 张泽[1] 赵晓航[1,2] 

机构地区：[1]海军总医院基础医学研究中心,北京100048 [2]中国医学科学院肿瘤医院分子肿瘤学国家重点实验室,北京100021

出　　处：《国际检验医学杂志》2018年第1期5-9,共5页International Journal of Laboratory Medicine

基　　金：国家高技术发展"863"计划资助项目(2012AA020206);国家自然科学基金资助项目(81372591;91629105)

摘　　要：目的探讨海上长远航作业环境对作业人员皮肤共生微生物的影响,以及皮肤共生微生物与宿主之间的相互关系。方法采集长远航作业人员作业前后脐周微生物样本,经16SrRNA V4区段扩增并测序和全宏基因组鸟枪法高通量测序;分析菌群结构、种类和微生物代谢功能改变;采集长远航作业与岸基作业人员外周血,进行血常规分析。结果海上航行105d后,长远航作业者脐周微生物群落多样性减小;硬壁菌门相对丰度升高,变形菌门相对丰度降低;葡萄球菌属相对丰度升高,而棒状杆菌属相对丰度降低,差异具有统计学意义(P<0.05)。特别是表皮葡萄球菌、金黄色葡萄球菌等致病菌或条件致病菌相对丰度显著增高。功能基因分析提示,长远航作业后期脐周微生物感染相关基因表达升高。与岸基作业人员相比,长远航作业组外周血淋巴细胞异常升高人员的比例显著增高,差异有统计学意义(P<0.05)。结论脐周皮肤共生微生物可能反映海上长远航作业者的健康状况。Objective To investigate the influence of long sea voyage working environment on the symbiotic microorganisms and their relationship with their hosts. Methods The periumbilical microbial sam-ples from the operating workers of long sea voyage before and after operation were collected. Then 16S rRNA V4 section amplification, sequencing and whole metagenome shotgun high-throughput sequencing were per-formed. Moreover the bacterial community structure,kinds and microorganism metabolic function change were analyzed. The peripheral blood was collected from the workers of long sea voyage operation and shore-based operation for conducting the blood routine analysis. Results After 105 d ocean sailing,the diversity of perium-bilical microbial community in the workers with long sea voyage operation decreased and the relative abun-dance of Firmicutes increased, while which of Proteobacteria decreased; which of Staphylococcus increased, while which of Corynebacteria decreased,the differences were statistically significant（P〈0. 05） .the relative a-bundance of pathogenic bacteria or conditional pathogenic bacteria, especially Staphylococcus epidermidis and Staphylococcusaureus aureus , increased significantly. The functional gene analysis indicated that the expres-sion of periumbilical microbial infection related genes increased after the long sea voyage operation. Compared with shore-based operation workers, the proportion of workers with peripheral blood lymphocytes abnormal elevation in the long sea voyage group increased significantly the difference was statistically significant （P〈0. 05）. Conclusion The periumbilical skin symbiotic microorganisms may reflect the health conditions in the workers with long sea voyage operation.

关 键 词：长远航 宏基因组 二代测序 皮肤微生物 

分 类 号：Q93-331[生物学—微生物学]