精液参数对体外受精助孕术后孕早期自发性流产的相关性分析  

Analysis on correlation between semen parameters with spontaneous abortion in early pregnancy after in vitro fertilization assisted pregnancy operation

作　　者：黎智彪[1] 黄莉[1] 黄晓卉[1] 刘艳香[1] 彭爱琴[1] 陈亮[1] 

机构地区：[1]南方医科大学广济医院生殖中心,广东东莞523690

出　　处：《国际检验医学杂志》2018年第1期49-51,55,共4页International Journal of Laboratory Medicine

摘　　要：目的探讨精液参数对体外受精助孕术后孕早期自发性流产的相关性。方法选择该院生殖中心行体外受精(IVF)助孕术后成功获得妊娠的220例患者作为研究对象,发生早期自发性流产40例患者作为观察组,未发生早期流产180例作为对照组。采用计算机辅助精子分析(CASA)对男方精液精子透明质酸未结合率、精子核蛋白不成熟率、精子形态正常率等相关参数指标进行测定分析。结果观察组精子-透明质酸未结合率、精子核蛋白不成熟率均显著高于对照组,差异有统计学意义(P<0.05);观察组精子形态正常率显著低于对照组,差异有统计学意义(P<0.05);两组患者精液其他参数指标水平比较,差异均无统计学意义(P>0.05)。经统计学分析,结果显示精子-透明质酸未结合率、精子DNA碎片率、精子核蛋白不成熟率这3项指标之间两两均呈正相关,与其他指标呈负相关。经二分类Logistic回归模型逐步分析,结果表明引起IVF术后孕早期自发性流产的高危因素包括精子核蛋白不成熟率及精子-透明质酸未结合率。结论精子核蛋白不成熟率及精子-透明质酸未结合率是诱发IVF术后孕早期自发性流产的两大高危因素。Objective To investigate the correlation between semen parameters with spontaneous abortion in early pregnancy after in vitro fertilization(IVF)assisted pregnancy operation.Methods 220 patients with successful pregnancy after IVF assisted pregnancy operation in the reproductive center of this hospital were selected as the research subjects.Among them,40 cases of early spontaneous abortion served as the observation group and 180 cases without early spontaneous abortion occurrence as the control group.The related parameters of sperm hyaluronic acid unbinding rate,sperm nucleoprotein immaturity rate and sperm morphology normal rate were measured and analyzed by adopting the computer assisted sperm analysis(CASA).ResultsThe sperm hyaluronic acid unbinding rate and sperm nucleoprotein immaturity rate in the observation group were significantly higher than those in the control group,the difference was statistically significant(P<0.05);the sperm morphology normal rate in the observation group was significantly lower than that in the control group,the difference was statistically significant(P<0.05);the other semen parameters had no statistical differences between the two groups(P>0.05).The statistical analysis result showed that the sperm hyaluronic acid unbinding rate,sperm DNA fragmentation rate and sperm nucleoprotein immaturity had the positive correlation between each other,which had the negative correlation with the other indicators.After the stepwise analysis by the binary Logistic regression model,the results showed that the high risk factors affecting spontaneous abortion in early pregnancy after IVF included sperm nucleoprotein immaturity rate and sperm hyaluronic acid unbinding rate.Conclusion The sperm nucleoprotein immaturity rate and sperm hyaluronic acid unbinding rate are two high-risk factors inducing early spontaneous abortion after IVF.

关 键 词：精液参数 体外受精助孕术 早期自发性流产 相关性 

分 类 号：R714.21[医药卫生—妇产科学]