铜绿假单胞菌痰培养阳性患者抗菌肽LL-37、CRP水平变化及临床意义  

Changes and clinical significance of antimicrobial peptide LL-37 and CRP in patients with Pseudomonas aeruginosa positive sputum culture

作　　者：肖倩[1,2] 黄柳霞 曾建明 李有强[1,2] 张伟铮 陈茶[1,2] 陈富 

机构地区：[1]广东省中医院检验医学部,广州510120 [2]广州中医药大学第二附属医院检验医学部,广州510006 [3]重庆医科大学医学检验系,重庆400016

出　　处：《国际检验医学杂志》2018年第2期146-148,共3页International Journal of Laboratory Medicine

基　　金：广东省医学科研基金项目(A2015457);广东省中医药局科研项目(20172075);广东省中医院中医药科学技术研究专项资助项目(YN2016QJ13)

摘　　要：目的探讨铜绿假单胞菌痰培养阳性患者的抗菌肽LL-37和C反应蛋白(CRP)水平变化情况及相互关系。方法选取广东省中医院2016年9月至2017年5月收治的铜绿假单胞菌痰培养阳性患者50例、健康体检27例作为研究对象,采用双抗体夹心酶联免疫吸附试验法检测抗菌肽LL-37水平,免疫比浊法检测CRP水平。结果铜绿假单胞菌痰培养阳性患者组的抗菌肽LL-37和CRP水平均显著高于健康对照组,差异有统计学意义(P<0.05),两者呈正相关(r=0.411,P<0.05)。结论铜绿假单胞菌痰培养阳性时,抗菌肽LL-37和CRP水平升高,对评估患者感染的早期诊断有一定临床应用价值。Objective To investigate the changes of antimicrobial peptide LL-37 and C-reactive protein （CRP） in the patients with Pseudomonas aeruginosa positive sputum culture and their mutual relation. Methods Fifty cases of Pseudomonas aeruginosa positive sputum culture and 27 cases undergoing physical examination in the Guangdong Provincial Hospital of Chinese Medicine from September 2016 to May 2017 were selected as the research subjects. The level of antimicrobial peptide LL-37 was detected by double antibody sandwich ELISA and the CRP level was detected by immunoturbidimetry. Results The levels of antimicrobial peptide LL-37 and CRP in the Pseudomonas aeruginosa positive sputum culture group were significantly higher than those in the healthy control group, the difference was statistically significant （P〈0. 05）, and they showed the positive correlation （r=0. 411 ,P〈0. 05）. Conclusion In positive sputum culture of Pseudomonas aeruginosa,antimicrobial peptide LL-37 and CRP levels are increased, which has a certain clinical application value for the early diagnosis of infection.

关 键 词：抗菌肽LL-37 C反应蛋白 铜绿假单胞菌 

分 类 号：R446.5[医药卫生—诊断学]