灌流液改性剂对环维黄杨星D微透析探针回收率的影响研究  

Influence of perfusate modifier on the microdialysis probe's recovery of cyclovirobuxine D

作　　者：刘新国[1] 汪娟[1] 陈晨 王胜麟 

机构地区：[1]武汉市第一医院,武汉430022

出　　处：《中南药学》2017年第12期1683-1686,共4页Central South Pharmacy

摘　　要：目的考察灌流改性剂对环维黄杨星D微透析探针回收率的影响,验证使用改性灌流液条件下微透析探针体外相对回收率与相对损失率的一致性,为体内微透析研究提供实验依据。方法采用高效液相色谱法测定灌流液中环维黄杨星D含量,计算不同改性灌流液条件下微透析探针的体外相对回收率,优选合适的改性剂种类与用量,再分别采用增量法与减量法计算不同药物浓度水平下探针体外相对回收率与相对损失率,比较两者结果的一致性。结果常规灌流条件下,乙醇作为灌流液改性剂对环维黄杨星D相对回收率提高较多,优选灌流液配比为30%乙醇-林格氏液。在2.53~10.12μg·mL^(-1)内环维黄杨星D体外相对回收率和相对损失率具有高度的一致性。结论选择合适的灌流液改性剂可显著提高微透析探针相对回收率水平,对于同一根微透析探针环维黄杨星D体外相对回收率与外周液药物浓度无关,探针回收率可通过药物透过半透膜时的流失量来间接计算,结果较好地证明反透析法适合于环维黄杨星D微透析采样。Objective To observe the influence of perfusate modifiers on the microdialysis probe recovery of cyclovirobuxine D, to verify the consistency between microdialysis probe relative recovery rate and relative loss rate under different experimental conditions, and to provide experimental evidence for the microdialysis research of cyclovirobuxine D in vivo. Methods The content of cyclovirobuxine D in perfusate samples was determinated by high performance liquid chromatography. The microdialysis probe relative recovery under different expenrimental conditions was calculated, to optimize the type and dosage of modifiers. The relative recovery rate and relative loss rate were calculated by the increment method and decrement method respectively. The two methods were consistent. Results Under normal experimental conditions, ethanol as the perfusion fluid increased the relative recovery rate more than others. The optimization of perfusate ratio was 30% ethanol-ringer solution. At 2.53 - 10.12 μg ·mL-1 concentration range, the cyclovirobuxine D relative recovery rate and relative loss rate in vitro were consistent. Conclusion The relative recovery rate of microdialysis probe may be significantly improved by selecting appropriate perfusate modifiers. For the same microdialysis probe, cyclovirobuxine D relative recovery has no association with the drug concentration of the peripheral liquid in vitro. The probe recovery rate can be calculated indirectly by drugs loss rate across the semipermeable membrane. The experimental results have proved that the reverse dialysis method is suitable for cyclovirobuxinum D microdialysis sampling.

关 键 词：环维黄杨星D 微透析 灌流液改性剂 探针回收率 

分 类 号：R285[医药卫生—中药学]