复方补骨脂酊的定性定量方法研究  

Qualitative and quantitative method for compound Buguzhi tincture

作　　者：李曙光[1] 刘志承[1] 曹丽萍 简晓莉[1] 徐汉明[1] 

机构地区：[1]深圳市中医院,广东深圳518033 [2]深圳市宝安中医院,广东深圳518133

出　　处：《中南药学》2017年第12期1771-1774,共4页Central South Pharmacy

摘　　要：目的建立复方补骨脂酊定性定量方法。方法采用TLC对复方补骨脂酊中补骨脂、白芷、丹参进行定性鉴别,采用HPLC进行含量测定,使用安捷伦TC-C_(18)色谱柱(250 mm×4.6 mm,5μm),乙腈-0.2%磷酸溶液为流动相进行梯度洗脱,检测波长246 nm,进样量10μL。结果薄层色谱斑点清晰,样品与对照药材和对照品在相应的位置上,分别显示相同颜色的荧光斑点;本品中补骨脂素、欧前胡素和丹参酮ⅡA平均含量分别为25.3425、10.1203、24.0054μg·mL^(-1),补骨脂素、欧前胡素和丹参酮ⅡA分别在10.4325~166.92μg·mL^(-1)(r=1.0000)、2.625~42μg·mL^(-1)(r=0.9998)及10.335~165.36μg·mL^(-1)(r=0.9999)与峰面积具有良好的线性关系,且平均加样回收率分别为99.9%(RSD=1.0%)、99.0%(RSD=0.80%)及99.7%(RSD=1.1%)。结论 TLC法可以用于本品补骨脂、白芷、丹参的鉴别,HPLC法可用于本品中补骨脂素、欧前胡素和丹参酮ⅡA的含量测定。Objective To establish a qualitative and quantitative method for compound Buguzhi tincture. Methods Psoralea corylifolia, Angelica dahurica, and Salvia miltiorrhiza were identified by TLC. Agilent TC-C18 column （250 mm × 4.6 mm, 5 9m） was used, the mobile phase was acetonitrile-0.2% phosphoric acid with a gradient elution mode, the detection wavelength was at 246 nm, and the injection volume was 10 μL.Results The TLC spots developed were clear, and the samples in the corresponding positions with the reference herb and the reference substance respectively. Psoralen, imperatorin and tanshinone Ⅱ A all had good linearity at 10.4325 - 166.92 μg· mL - 1 （r = 1.0000）, 2.625 - 42 μg · mL - 1 （r = 0.9998）, and 10.335 - 165.36 μg · mL - 1 （r = 0.9999）. The average recoveries were 99.9% （RSD = 1.0%）, 99.0% （RSD = 0.80%） and 99.7% （RSD = 1.1%）, correspondingly. Conclusion The method is reliable in the identification of Psoralea corylifolia, Angelica dahuriea and Salvia miltiorrhiza in compound Buguzhi tincture. The method is accurate and reliable in the determination ofpsoralen, imperatorin and tanshinone Ⅱ A in compound Buguzhi tincture.

关 键 词：复方补骨脂酊 高效液相色谱法 薄层色谱法 补骨脂素 欧前胡素 丹参酮ⅡA 

分 类 号：R917[医药卫生—药物分析学]