近交系长爪沙鼠生化标记遗传监测方法的建立及其应用  

Establishment and Application of Genetic Monitoring Methods for Inbred Gerbil by Biochemical Markers

作　　者：李迎 陈振文[3] 马兰芝 尚世臣 尚玉璞 赵权[2] 李桂军 崔晓霞 王冬平 杜小燕[3] 

机构地区：[1]军事科学院军事医学研究院实验动物中心,北京100071 [2]吉林农业大学动物科学技术学院,长春130118 [3]首都医科大学实验动物科学部,北京100069

出　　处：《实验动物与比较医学》2017年第6期442-447,共6页Laboratory Animal and Comparative Medicine

基　　金：国家科技支撑计划课题(2015BA109B01-04)

摘　　要：目的建立近交系长爪沙鼠生化标记遗传检测方法,开展对长爪沙鼠脑缺血模型CMU/1和CMU/2近交系的纯合度进行分析,为判定其遗传状况提供依据。方法采用醋酸纤维板电泳法,选26个生化标记进行近交系长爪沙鼠各组织器官样品电泳,参考前期方法优化最佳电泳条件,并对样品处理和染色的方法进行一定的改良后;针对F21～23代77只CMU/1和44只CMU/2近交系动物26个生化标记的纯合度进行检测。结果在2个近交系长爪沙鼠共121只动物中均能成功检测到26个生化标记。其中有24个位点在CMU/1和CMU/2品系内和品系间均显示单态性,但是Es-3和Es-4位点在2个品系间有差异。结论建立了长爪沙鼠近交系生化标记遗传检测方法;确定近交系CMU/1和CMU/2动物26个生化标记,其纯合度达到100%,说明2个近交系品系符合近交系动物的标准(GB14923-2010)。Objective To establish genetic monitoring method by biochemical markers for inbred gerbils and apply it in analyzing the homogenous of the ischemia-prone inbred gerbil lines CMU/I and CMU/2. Methods Twenty-six biochemical marker loci were selected to perform cellulose acetate fiber electrophoresis for several kinds of gerbil tissues by optimum electrophoresis conditions referred to previous report and optimized the sample treatment and staining method. Then these methods were used in detecting homogeneous of 2 inbred lines CMU/1 （77 gerbils） and CMU/2 （44 gerbils） genetic quality involved generation F21-F23. Results All of 26 biochemical marker loci could be detected success- fully in both inbred gerbil of 121 gerbils. Thereinto, 24 loci exhibited monomorphism within and between CMU/1 and CMU/2. However, the loci Es-3 and Es-4 showed polymorphism between two strains. Conclusion The biochemical marker method for genetic monitoring of inbred gerbil has been successfully established. The 26 biochemical marker loci for inbred gerbil strain CMU/1 and CMU/2 has been confirmed which homogeneous reached to 100%. These data indicated that two inbred strains match the standard of inbred laboratory animals （GB 14923-2010）.

关 键 词：长爪沙鼠 近交系 脑缺血模型 生化位点标记 遗传监测 

分 类 号：Q95-33[生物学—动物学]