高油酸油菜脂肪酸代谢的蛋白质组学与转录组学关联分析  

Transcriptomics and Proteomics Correlation Analysis of Fatty Acid Metabolism in High Oil Rape

作　　者：王晓丹[1] 肖钢[1] 常涛 张振乾[1] 官春云[1] 邬贤梦[1] 

机构地区：[1]湖南农业大学农学院,南方粮油作物协同创新中心,湖南长沙410128

出　　处：《华北农学报》2017年第6期31-36,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家“973”计划项目(2015CB150206);国家自然科学基金项目(31201240);湖南省科技重大专项(2014FJ1006);作物种质创新与资源利用国家重点实验室培育基地开放课题;湖南农业大学作物学开放基金项目(ZWKF201302)

摘　　要：为了解高油酸油菜脂肪酸代谢的调控机理,以一组高油酸近等基因系自交授粉后20~35 d的种子为材料,进行同位素相对标记技术与绝对定量技术关联转录组分析。结果表明:蛋白质组和转录组相关性并不高,定量蛋白和基因关联系数为-0.326 9;变化趋势相反差异蛋白和基因的表达关联系数为-0.735 8;变化趋势相同差异蛋白和基因的表达关联系数为0.714 3。此外,鉴定出与差异蛋白表达趋势相同的差异基因80个,GO注释表明,这些基因涉及代谢、信号转导、防御与胁迫应答、氧化还原、转录等方面的功能,其中与脂肪酸代谢过程相关的有23个,上调表达16个,下调表达7个。gi|297330358(庚二酰ACP甲基酯的羧酸酯酶)、gi|297321940(磷酸化酶)、gi|297314818(乙酰胺、甲酰胺酶家族)可能在高油酸油菜脂肪酸代谢中发挥重要作用。证明蛋白质组学与转录组学关联分析能够对研究高油酸油菜脂肪酸代谢机制提供新思路。In order to study the regulation mechanism of fatty acid metabolism in high oleic rapeseed,a group of highly oleic acid near-isogenic lines was used as the material from 20 to 35 days after pollination,and was discussed by isobaric tags for relative and absolute quantitation with transcriptome technology.The results showed that the correlation coefficient between protein and transcript group was not high,and the correlation coefficient between quantitative protein and gene was-0.326 9.The correlation coefficient of differential protein and gene expression was-0.735 8,and the correlation coefficient of identical protein and gene expression was 0.714 3.Besides,80 differentially expressed genes were identified,GO annotation suggested that these genes involved in metabolism,signal transduction,defense and stress responses,redox,transcription,and so on,23 of the 80 genes were associated with fatty acid metabolism,16 of them were up-regulated and 7 down-regulated.gi ｜ 297330358（pantolactone ACP methyl ester）,gi ｜ 297321940（phosphorylase） and gi ｜ 297314818（acetamide/formamidase family） may play an important role in the case of high oleic acid rapeseed fatty acid metabolism.This study shows that proteomics and transcriptase association analysis can provide a new idea for the mechanism of fatty acid metabolism in high oil rape.

关 键 词：蛋白质组学 转录组学 关联分析 高油酸油菜 脂肪酸代谢 

分 类 号：S565.03[农业科学—作物学] Q78[生物学—分子生物学]