水稻耐盐性QTL的meta分析及候选基因发掘  

Meta-analysis of Salt Tolerance QTLs and Mining of Candidate Genes

作　　者：雷蕾[1] 郑洪亮[1] 杨洛淼 赵宏伟[1] 王敬国[1] 刘化龙[1] 孙健[1] 邹德堂[1] 

机构地区：[1]东北农业大学水稻研究所,黑龙江哈尔滨150030

出　　处：《华北农学报》2017年第6期45-53,共9页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(31601377);黑龙江省重大科技招标基金项目(GA14B102-02)

摘　　要：为了获得真实有效的水稻耐盐性QTL信息,并缩小QTL的置信区间以及预测区间内的候选基因。以水稻6个遗传图谱的整合结果为公共图谱,并收集344个与水稻耐盐性相关的QTL。利用Bio Mercator V4.2,在图谱整合的基础上,对一致性QTL区间的注释基因进行生物信息学分析。结果表明:共检测到46个和水稻耐盐性相关的一致性QTL,对其中8个物理距离在0.5 Mb附近的一致性QTL区间进行了物理图谱定位和候选基因分析,共有1 489个预测基因被检测到,参与了细胞合成、催化活性、水解酶活性和新陈代谢等主要功能。对一致性QTL区间内已克隆的水稻耐盐基因进行蛋白序列分析后,发现9个候选基因同已知耐盐基因的保守结构域相似,分别为OS01G0813100、OS01G0869900、OS02G0753000、OS03G0282100、OS03G0607700、OS07G0150500、OS09G0369050、OS09G0434500和OS09G0522100。研究发现的遗传距离小于0.1 c M的Mqtl两端的标记可用于分子标记辅助育种,发掘的9个和水稻耐盐基因具有高度蛋白序列相似性的注释基因,可为水稻耐盐性基因的克隆和功能验证奠定基础。In order to obtain the effective information of QTLs related to salt tolerance in rice from previous studies,the purpose of mining candidate genes in interval of QTLs was realized.Six rice genetic maps were integrated and 344 QTLs related to salt tolerance of rice were collected.The collected QTLs were analyzed by Bio Mercator V4.2 software,and then the bioinformatics analysis was performed on the QTLs of the consistent QTLs.The results showed that 46 QTLs related to salt tolerance of rice were detected.8 QTLs with physical distance close to 0.5 Mb were analyzed by physical map and candidate gene analysis.A total of 1 489 genes were found,involved in cell synthesis,catalytic activity,hydrolase activity and metabolism and other major functions.9 rice salt tolerance candidate genes were discovered by comparatively analyzing the salt tolerance gene from the consensus QTL region,those 9 candidate genes（OS01G0813100,OS01G0869900,OS02G0753000,OS03G0282100,OS03G0607700,OS07G0150500,OS09G0369050,OS09G0434500 and OS09G0522100） have the same conserved domain with salt tolerance gene.The results showed that the markers with Mqtl at the genetic distance of less than 0.1 cM could be used for the molecular marker-assisted breeding; 9 annotated genes with high protein sequence similarity to salt tolerance genes in rice were found.These candidate genes could lay the foundation for cloning and functional verification of salt tolerance genes in rice.

关 键 词：水稻 耐盐性状 元分析 候选基因 

分 类 号：Q37[生物学—遗传学] Q78