GLP试验中常用大鼠采血方法的探讨  

Exploration on Common Methods of Drawing Blood in Rats in GLP Studies

作　　者：王超[1] 王铎 秦超 李明[1] 耿兴超[1] 

机构地区：[1]中国食品药品检定研究院安全评价研究所药物非临床安全性评价研究北京重点实验室,北京100176

出　　处：《实验动物科学》2017年第6期58-60,共3页Laboratory Animal Science

基　　金："十二五"重大新药创制专项课题(No.2015ZX09501004-002)

摘　　要：目的探讨GLP试验条件下的大鼠不同采血方法及优缺点,保证GLP试验的顺利开展。方法使用2 100只大鼠,分别通过眼眶静脉丛采血法、颈外静脉采血法、腋窝静脉采血法和腹主静脉采血法四种方法进行采血试验。结果眼眶后静脉丛采血法首次采血成功率95.2%,操作简单快速,但易污染。颈静脉采血法首次采血成功率89.3%。腋窝静脉采血法首次采血成功率82.8%,可作为颈静脉采血法的补充。腹主静脉采血法成功率100%,操作简单,采血量大。结论在GLP试验中,应根据不同的要求灵活选用或组合采血方法,保证完成试验的同时又满足动物福利的要求。Objective Compare and explore the four method of drawing blood in rats to provide reference for selecting appropriate method in GLP studies. Method Blood samples were collected from 2 100 rats with four method , including orbital venous plexus, external jugular vein, axillary vein and abdominal vein blood collections. Result The success rate of first blood collection from post-orbital venous plexus was 95.2%. The operation is simple and fast, but is easy to be polluted. The success rate of first blood collection from jugular vein was 89.3%. The success rate of first blood collection from axillary vein was 82.8%. This method can be used as a supplement of jugular vein blood sampling. The success rate of abdominal main vein blood collection was 100% , and the operation was simple and with large blood volume. Conclusion All kinds of method should be combined flexibly according to the requirements of the experiment and animal welfare in GLP studies.

关 键 词：大鼠 GLP试验 采血方法 

分 类 号：Q95-33[生物学—动物学]