人胎膈腹膜间皮细胞的冷冻复型电镜观察  

Electron Microscopic Study of Mesothelial Cells in the Diaphragm Peritoneum of Human Fetus Using the Freeze-fracture Replica Method

作　　者：李继承 

机构地区：[1]浙江分院

出　　处：《中国医学科学院学报》1991年第3期189-194,共6页Acta Academiae Medicinae Sinicae

基　　金：国家自然科学青年基金

摘　　要：应用冷冻复型法对人胎隔腹膜间皮细胞作电镜观察。结果表明:(1)间皮细胞内拥有大量小泡,可单个或成簇存在;后者常融合成大泡,开口于细胞游离面,形成分泌颗粒。(2)在其核膜和细胞质膜不同的劈裂面,膜微粒的数目不同。细胞质膜的PF面有外排小孔,直径43nm,最集中处达26个/μm^2。(3)相邻细胞以指状胞质突起相连,形成立体镶嵌。(4)间皮细胞微绒毛有3种形态,即分叉状微绒毛,鼓槌状微绒毛和棒状微绒毛。此外,同一样本的超薄切片透射电镜观察也支持以上形态学特征。Electron microscopy of mesothelial cells from the diaphragm peritoneum of human fetuses was conducted using the freeze-fracture replica method. The results were as foliows:1) Numerous vesicles were found in single or cluster forms. The latter type was often fused with large vacuoles and opened to the free surface of the cell as secretory granules. 2) On the fractured faces of plasma and nuclear membranes, different numbers of intramembraneous particles and exocytic pores were seen on the PF surface of the plasma membrane. The diameter of the exocytic pores averaged 43 nm, and their maximum number was 26/μm2.3) Neighboring cells were conjoined by cytoplasmic processes which were seemd to form three-dimensional intertwinings. 4) The microvilli of the mesothelial cells were grouped into three morphological classes, i.e. fork-like microvilli, bulbous ending microvilli, and stick-like microvilli. In addition, the contrast between the present investigation and the results of fine section studies on the diaphragm peritoneum of human fetus is discussed.

关 键 词：间皮细胞 膈腹膜 超微结构 人胎 

分 类 号：R329.27[医药卫生—人体解剖和组织胚胎学]