包埋脱水法常温和低温保存裙带菜配子体的研究  

Storage of Gametophytes of Undaria by Encapsulation-dehydration at Normal Temperature and Low Temperature

作　　者：刘艳萍[1] 张岚翠[2] 王起华[2] 

机构地区：[1]塔里木大学植物科学学院,新疆阿拉尔843300 [2]辽宁师范大学生命科学学院,辽宁大连116029

出　　处：《塔里木大学学报》2017年第4期59-63,共5页Journal of Tarim University

基　　金：国家自然科学基金资助项目(30170099;30470184;31160069)

摘　　要：采用包埋脱水法在20℃、4℃和-20℃条件下对裙带菜配子体进行保存,研究了保存温度、胶球含水量以及光照等因素对裙带菜配子体存活率的影响。含水量为50%时配子体在20℃/暗和4℃/暗获得了较好的存活率,保存360 d后存活率分别为22.4%和56.3%。保存360 d的裙带菜配子体可以发育成幼孢子体,且与对照相比无明显差异。结果表明,该方法适合对裙带菜配子体进行中长期保存,该方法操作简单,对裙带菜的保种及育苗有重要意义。Gametophytes of Undaria pinnatifida were preserved by encapsulation-dehydration at 20 ℃,4 ℃ and-20 ℃ to determine the optimal temperature. The results showed that the high viability were obtained under beads water content of 50% at 20 ℃/dark and 4 ℃/dark. After 360 days,the viability could reach about 22. 4% at 20 ℃/dark,56. 3% at 4 ℃/dark. After storage for 360 days,the gametophytes could produce normal sporophytes in the conditions suitable for development. The results indicated that encapsulation-dehydration method is suitable for preservation of the gametophytes,since it is simple in operation which would have important significance for the conservation and breeding of Undaria pinnatifida.

关 键 词：裙带菜配子体 包埋脱水法 保存 

分 类 号：Q-33[生物学]