Development of a stable SCAR marker for rapid identification of Ganoderma lucidum Hunong 5 cultivar using DNA pooling method and inter-simple sequence repeat markers  

作　　者：CHAO Wen-zheng TANG Chuan-hong ZHANG Jing-song YU Ling Honda Yoichi 

机构地区：[1]School of Perfume and Aroma Technology,Shanghai Institute of Technology [2]Key Laboratory of Edible Fungus Resources and Utilization (South),Ministry of Agriculture/National Engineering Research Center of Edible Fungi/National R&D Center for Edible Fungi Processing/Key Laboratory of Agriculture Genetics and Breeding of Shanghai,Institute of Edible Fungi,Shanghai Academy of Agricultural Sciences [3]Laboratory of Forest Biochemistry,Graduate School of Agriculture,Kyoto University

出　　处：《Journal of Integrative Agriculture》2018年第1期130-138,共9页农业科学学报（英文版）

基　　金：financially supported by the National Natural Science Foundation of China (31401933);the Shanghai Municipal Committee of Agriculture,China (G2014070107)

摘　　要：The cultivar Ganoderma lucidum Hunong 5 was obtained using cross-breeding. Hunong 5 has high commercial value due to its high polysaccharide and triterpene content, This is the first report of using a DNA pooling method to develop a stable sequence characterized amplified region （SCAR） marker for rapid identification of the G. lucidum Hunong 5 cultivar. The SCAR marker was developed by first generating and sequencing a distinctive inter simple sequence repeat （ISSR） fragment （882 bp） from G. lucidum Hunong 5 cultivar. A stable SCAR primer pair GLH5F/GLH5R were obtained to identify the cultivar and the SCAR marker is a DNA fragment of 773 bp.The cultivar Ganoderma lucidum Hunong 5 was obtained using cross-breeding. Hunong 5 has high commercial value due to its high polysaccharide and triterpene content, This is the first report of using a DNA pooling method to develop a stable sequence characterized amplified region （SCAR） marker for rapid identification of the G. lucidum Hunong 5 cultivar. The SCAR marker was developed by first generating and sequencing a distinctive inter simple sequence repeat （ISSR） fragment （882 bp） from G. lucidum Hunong 5 cultivar. A stable SCAR primer pair GLH5F/GLH5R were obtained to identify the cultivar and the SCAR marker is a DNA fragment of 773 bp.

关 键 词：DNA pooling Ganoderma lucidum Hunong 5 cultivar ISSR marker SCAR marker 

分 类 号：S0[农业科学]