女贞子炮制前后环烯醚萜苷类成分转化机制研究  

Research on Transformation Mechanism of Secoiridoid Glycosides Components in Ligubtrum lucidum Before and After Processing

作　　者：张学兰[1,2] 宋梦晗 姜秋 李慧芬[1,2] 徐保鑫[1] 栾茹乔 刁家葳 

机构地区：[1]山东中医药大学,山东济南250355 [2]国家中医药管理局中药炮制技术传承基地,山东济南250355

出　　处：《辽宁中医杂志》2017年第12期2602-2604,共3页Liaoning Journal of Traditional Chinese Medicine

基　　金：国家自然科学基金面上项目(81373968);国家公共卫生专项中药炮制技术传承基地建设项目(2015)

摘　　要：目的:探讨女贞子蒸制过程中环烯醚萜苷类成分的转化机制。方法:采用LC-TOF/MS法,通过对照品定位、质谱数据、文献参照,分析女贞子4种环烯醚萜苷类化合物(oleopolynuzhenide A、oleonuezhenide、女贞苷G13、女贞次苷)模拟清蒸品的化学成分。液相色谱采用Diamonsil C18柱(2.1 mm×150 mm,5μm),流动相为A(乙腈)-B(0.1%甲酸溶液),梯度洗脱,检测波长240 nm;质谱使用Agilent 6230 LC-TOF/MS联用系统,Dual ESI离子源,在ESI/MS正离子模式下采集数据。结果:4种环烯醚萜苷类化合物模拟清蒸品中均检出了橄榄酸,oleonuezhenide模拟清蒸品中还检出了特女贞苷、红景天苷和特女贞苷异构体,女贞次苷模拟清蒸品中还检出了红景天苷和女贞次苷异构体。与oleonuezhenide模拟清蒸品成分比较,女贞苷G13模拟清蒸品的成分增加了女贞次苷和女贞次苷异构体,oleopolynuzhenide A模拟清蒸品的成分增加了oleonuezhenide、女贞苷G13、女贞次苷和女贞次苷异构体。结论:oleopolynuzhenide A、oleonuezhenide、女贞苷G13、女贞次苷性质不稳定,加水、加热可使其水解生成其次级苷和苷元。女贞子酒蒸后补肝肾作用增强与女贞子中环烯醚萜苷类成分在炮制过程中发生水解转化密切相关。Objective：To discuss the mechanism of transformation of secoiridoid glycosides under steaming time spans with wine. Methods ： By reference positioning, mass spectrometry data, literature reference, we analyzed the chemical components of the four kinds of secoiridoid glycosides simulated steamed products under LC - TOF/MS technology. An HPLC method was applied with a Diamonsil Cls column （2.1 mm ~ 150 ram,5 p^m） ,A （acetonitrile） - B （0. 1% formic acid solution） as mobile phase with gradient elution and the detection wavelength was 240 nm. The mass spectrometry was Agilent 6230 LC - TOF/MS coupling system and Dual ESI ion source and was performed in the positive ion mode to collect data. Results ： Elcnolic acid was detected in the four kinds of secoiridoid glycosides simulated steamed products. The oleonuezhenide simulated steamed product also detected specnuezhenide, salidroside and specnuezhenide isomers. The glucosylformoside simulated steamed product also detected salidro- side and glucosylformoside isomer. Compared with oleonuezhenide simulated steamed product,ligustroside G13 simulated steamed product detected glucosylformoside and glueosylformoside isomer. The oleopolynnzhenide A simulated steamed product detected oleopolynuzhenide ,ligustroside G13, glucosylformoside and glucosylformoside isomer. Conclusion：The four kinds of secoiridoid gly- cosides in Ligubtrum lucidum Ait are instable. Water or heating can make it hydrolysis to produce its secondary glycosides or glycosides. The enhancement of tonifying liver and kidney efficacy is closely related to hydrolysis of its secoiridoid glycosides.

关 键 词：女贞子 环烯醚萜苷类成分 转化机制 LC-TOF/MS 

分 类 号：R284[医药卫生—中药学]