基于富G碱基单链DNA构建荧光生物传感器应用于铽离子的检测  

Construction of fluorescence biosensor based on single-stranded G-rich base DNA and application on the detection of terbium ions

作　　者：陈家越[1] 陈强 

机构地区：[1]福建船政交通职业学院安全技术与环境工程系,福建福州350003 [2]福建省环境监测中心站,福建福州350003

出　　处：《宁德师范学院学报（自然科学版）》2017年第4期372-377,384,共7页Journal of Ningde Normal University(Natural Science)

基　　金：福建省交通厅科技资助项目(201323);福建省工业科技重点项目(2012H0005);福建省环保科技计划项目(2016R014)

摘　　要：选择四条具有代表性的DNA序列,分别考察在铽离子作用下DNA构型变化对荧光的增敏效果.实验表明,相对于平行或反平行结构,无自折叠DNA单链结构对铽离子的荧光增敏效果最佳.在最佳条件下,DNA单链结构对铽离子检测线性范围为6.0×10-2~6.0μmol·L-1,检出限为8.0×10-3μmol·L-1.Four representative DNA sequences were selected to investigate the effect of Tb3~ on DNA structures and fluorescence sensitizations, and then the fluorescence mechanism of G-quadruplex sensitized Tb3＋ were explored. The results showed that non-self-folding DNA configuration had the best effect of sensitization, compared to the DNA antiparallel or parallel structures. Non-self-folding DNA configuration was then chosen for Tb3＋ detection, the linear range was 6.0×10^-2-6.0μmol·L-1, and the detection limit was 8.0×10^-3 μmol·L-1.

关 键 词：富G碱基DNA 铽离子 G-四倍体 荧光 

分 类 号：TP212.3[自动化与计算机技术—检测技术与自动化装置]