积雪草苷在SD大鼠体内组织分布的研究  

Study on the Tissue Distribution of Asiaticoside in SD Rats

作　　者：朱丽莎 刘颖菊[2] 谭欣玮[2] 

机构地区：[1]南充市中心医院.川北医学院第二临床学院临床药学科,四川南充637000 [2]重庆医科大学药学院药理教研室/重庆市生物化学与分子药理学重点实验室

出　　处：《中国药师》2018年第1期20-22,27,共4页China Pharmacist

基　　金：留学回国人员科研启动基金项目(编号:2006-331)

摘　　要：目的:研究积雪草苷在SD大鼠体内的组织分布情况。方法:尾静脉注射SD大鼠有效治疗剂量42 mg·kg^(-1),于5,30,80 min将大鼠拉断脊椎处死,立即解剖取出心、肝、脾、肺、肾、胃、肠、脑、生殖腺、左大腿肌肉组织,采用HPLC法测定各组织中积雪草苷的含量。结果:积雪草苷在SD大鼠体内分布广泛,其中心浓度最高,其次是肝、脑、肺、脾、肾,其他组织相对较低。结论:本研究方法方便、灵敏、准确,可用于大鼠组织中积雪草苷含量的测定。Objective： To investigate the tissue distribution of asiaticoside in SD rats. Methods： The rats were injected by asiatico- side at the effective treatment dose of 42 mg kg- t via tail vein. The rats sacrificed respectively at 5, 30 and 80 min after the injection and heart, liver, spleen, lung, kidney, stomach, intestine, brain, gonad and left thigh muscle were dissected immediately. The con- tent of asiaticoside in the tissues was determined by HPLC. Results： Asiaticoside widely distributed in SD rats, and the concentration in heart was the highest followed by liver, brain, lung, spleen and kidney. The contents in the other tissues were relatively low. Con- clusion： The method is convenient, sensitive and accurate, and can be used for the content determination of asiaticoside the tissues of SD rats.

关 键 词：积雪草苷 组织分布 SD大鼠 

分 类 号：R945[医药卫生—微生物与生化药学]