肥胖抑制素减轻高糖致大鼠INS1细胞的凋亡  

Obestatin attenuates apoptosis induced by high glucose in INS1 cells

作　　者：徐敏 王威[1] 姜方旭 乔虹[1] 张玲[1] 

机构地区：[1]哈尔滨医科大学附属第二医院内分泌与代谢病科,黑龙江哈尔滨150086 [2]西澳大学Harry Perkins医学研究院,尼德兰兹WA6009 [3]郑州市第三人民医院内分泌科,河南郑州450000

出　　处：《基础医学与临床》2018年第1期7-12,共6页Basic and Clinical Medicine

基　　金：国家自然科学基金(81471081);黑龙江省自然科学基金(H201416);黑龙江省教育厅科学技术研究项目(11551199);哈尔滨医科大学附属第二医院博士基金(BS2011-12)

摘　　要：目的探讨肥胖抑制素(OB)减轻高糖致大鼠胰岛细胞系INS1细胞的凋亡作用。方法 INS1细胞在不同糖浓度环境下培养24 h,用MTT法检测INS1细胞存活率和增殖;Hoechst33258细胞核染色法检测细胞核形态学;酶标法检测caspase-3活性及OB的保护作用是否涉及了PI3K;real-time PCR检测FOXO1、SREBP1c、Bax和PDX-1mRNA的表达。结果在高糖条件下,OB促进INS1细胞的增殖,在100 nmol/L浓度时能最大程度促进INS1细胞增殖(与对照组、高糖组相比,P<0.01)。OB能减轻高糖诱导的凋亡(P<0.01);在高糖组,FOXO1、SREBP1c和Bax基因表达较对照组增多,PDX-1表达减少;反之在OB干预的高糖组;FOXO1、SREBP1c和Bax表达减少,PDX-1表达增多。结论 OB能够减轻高糖致大鼠INS1细胞的损伤作用。Objective To investigate the effect of obestatin on the apoptosis of rat pancreatic islet cell line INS1 induced by high glucose. Methods INS1 cells were cultured in different concentrations of glucose. The survival rate and proliferation of INS1 cells were detected by MTT method; Hoechst33258 nuclear staining was used to detect nuclear morphology. caspase-3 method was used to study the relationship between the protective effect of obestatin and the PI3 K pathway; Finally,using real-time PCR detection of FOXO1 and SREBP1c,Bax,PDX-1 expression,to further clarify the protective effect of obestatin on cells. Results In high glucose condition,obestatin promoted the proliferation of INS1 cells at 100 nmol/L,and promoted the proliferation of INS1 cells significantly（ P〈0. 01,compared with the control group and high glucose group）.Obestatin can reduce high glucose-induced apoptosis（ P 0. 01）. The expressions of FOXO1,SREBP1 c,Bax and PDX-1 decreased,while the expression of FOXO1,SREBP1c,Bax and PDX-1 increased in high glucose group. Conclusions OB can attenuate the injury of INS1 cells induced by high glucose in rats.

关 键 词：肥胖抑制素 糖毒性 INS1细胞 保护作用 

分 类 号：R58[医药卫生—内分泌]