慢性阻塞性肺疾病大鼠肺组织中肺表面活性物质蛋白-C表达下降  

Decreased expression of surfactant protein C in lung tissue of chronic obstructive pulmonary disease rats

作　　者：张伟[1] 王煜霞[1] 朱认真 姬明丽[1] 

机构地区：[1]新乡医学院基础医学院,河南新乡453003

出　　处：《基础医学与临床》2018年第1期47-50,共4页Basic and Clinical Medicine

基　　金：河南省科技厅基础与前沿技术研究计划(132300410160);河南省科技厅科技攻关研究计划(162102310121);新乡医学院高学历人才启动基金(505001)

摘　　要：目的探讨肺表面活性物质蛋白-C(SP-C)在慢性阻塞性肺疾病(COPD)大鼠肺组织中的作用。方法将大鼠随机分为对照组、香烟烟雾暴露组、脂多糖组和COPD组,每组10只。测定各组大鼠的PaO_2和PaCO_2的水平;透射电镜观察肺组织的细胞微观结构;ELISA检测支气管肺泡灌洗液(BALF)和肺组织SP-C蛋白;RT-q PCR检测肺组织SP-C mRNA的表达。结果与其他组相比,COPD大鼠的PaO_2最低,而PaCO_2最高;肺泡Ⅱ型上皮细胞表面微绒毛明显减少(P<0.01);BALF和肺组织中SP-C蛋白表达下降(P<0.01);肺组织中的SP-C mRNA表达下降(P<0.01)。结论 SP-C在COPD大鼠肺组织中表达下调,这种下调可能引起肺通气和肺换气功能障碍。Objective To study the role of surfactant protein C（ SP-C） in rat lung of chronic obstructive pulmonary disease（ COPD）. Methods Forty healthy conventional Sprague-Dawley（ SD） rats were randomly divided into four groups,normal control group（ control group）,smoke exposure group（ smoking group）,lipopolysaccharide group（ LPS group）,smoke exposure ＋ Lipopolysaccharide group（ COPD group）. The arterial partial pressure oxygen（ PaO_2） and arterial partial pressure of carbon dioxide pathological（ PaCO_2） were detected. The ultrastructure of lung tissue was observed by transmission electron microscope. Enzyme-linked immuno sorbent assay（ ELISA） were performed to determine protein expression of SP-C in lung and bronchoalveolar lavage fluid（ BALF）. RT-q PCR were performed to determine mRNA expression of SP-C in lung. Results Compared with control group,smoking group and LPS group,the PaO_2 of COPD group was obviously lower,the PaCO_2 of COPD group was obviously higher; the ultrastructure and histological analysis of lung tissues showed chronic inflammatory injury; Compared with control group,the expression of SP-C protein in was reduced,as well as SP-C mRNA expression. Conclusions The expression of SP-C in lung of rats COPD model is down-regulated. SP-C may be involved in COPD.

关 键 词：慢性阻塞性肺疾病 表面活性物质蛋白-C 呼吸功能 

分 类 号：R994.6[医药卫生—毒理学]