2013-2015年福建省PRRSV ORF3基因亚型遗传变异分析  

Genetic variations of ORF3 gene of PRRSV in Fujian province from 2013 to 2015

作　　者：魏春华[1] 刘建奎[1] 戴爱玲[1] 谢莹 杨小燕[1] 

机构地区：[1]龙岩学院生命科学学院福建省家畜疫病防治与生物技术重点实验室福建省生猪疫病防控工程技术研究中心

出　　处：《中国兽医学报》2018年第1期17-24,共8页Chinese Journal of Veterinary Science

基　　金：福建省科技重大专项资助项目(2014NZ0002-3);福建省省属高校科研课题资助项目(JK2015049);福建省自然科学基金资助项目(2016J01168);福建省科技平台资助项目(2014N2011)

摘　　要：为了研究2013—2015年福建省猪繁殖与呼吸综合征病毒（PRRSV）的流行特点及变异规律，收集福建多个地区的26株PRRSV分离株，应用RT-PCR方法对其ORF3基因进行克隆及序列分析，从而了解福建地区PRRSV的遗传变异性。结果表明，26株毒株中24株为美洲型毒株（NAgenotype，type2），2株为欧洲型毒株（EUgenotype，type 1）。26株PRRSVORF3核苷酸同源性为63．1％～100％，与VR2332的同源性为64．8％～99．5％，与HP-PRRSV代表毒株JXA1、WuH1和TJ同源性为63．7％～99．8％，与NADC30同源性为66．1％～95．8％，与欧洲代表毒株LV的同源性为63．6％～90．9％。24株美洲型ORF3遗传进化树表明，福建地区PRRSV出现了2个新的亚群，3亚群（2株处于独立的分支）和4亚群（3株为类NADC30PRRSV）。欧洲型PRRSVORF3遗传进化树表明，福建地区流行的毒株为泛亚1型毒株。综上所述，福建地区PRRSV存在2种血清型毒株，美洲型流行毒株呈现遗传变异多样性。In order to research the molecular biological characteristics and variation rule of prevalent porcine reproductive and respiratory syndrome virus （PRRSV） in Fujian province during 2013-- 2015. Twenty-six PRRSV isolates from Fujian were collected, ORF3 genes were cloned and sequenced by RT-PCR in order to understand genetic variability of PRRSV in Fujian province. The result showed that, among 26 isolates 24 belonged to the North American genotype （type 2 PRRSV） ,2 isolates belonged to the European genotype （type 1 PRRSV）. The nucleotide identity among twenty-six isolates ranged from 63. 1%-100%, shared 64. 8% -99. 5% homology with VR2332;63. 7%-99. 8% with JXAI,WUH1 and TJ;66. 1%-95. 8%. with NADC30 and 63. 6%- 90.9% with LV. The phylogenetic tree analysis showed that there were two new suhgenotypes in these Fujian PRRSV strains,subgroup Ⅲ （two isolates formed a new subgenotype ） and subgroup Ⅳ （three isolates formed NADC30 PRRSV）. The genetic evolution tree of European type PRRSV ORF3 showed that the prevalent strain in Fujian was Fan Asian type 1 strain. These results showed that type land type 2 coexisted in the Fujian province and type 2 PRRSV have been shown a remarkable genetic diversity.

关 键 词：猪繁殖与呼吸综合征病毒 ORF3基因 RT—PCR 遗传变异 

分 类 号：S852.65[农业科学—基础兽医学]