恒山仿野生黄芪绝对生长年限鉴别及黄酮和皂苷积累规律研究  

Identification of growth years of Hengshan imitative wild culture Astragali Radix and investigation into the accumulation rules of flavonoids and saponins

作　　者：高四云 李科[1,2,3] 熊一峰[1,4,2,3] 秦雪梅 崔贵梅[5] 张翔[6] 杜冠华[7] 

机构地区：[1]山西大学中医药现代研究中心,山西太原030006 [2]山西大学中药功效物质与新药创制山西省重点实验室,山西太原030006 [3]山西大学化学生物学与分子工程教育部重点实验室,山西太原030006 [4]山西大学化学化工学院,山西太原030006 [5]山西省农业科学院生物技术研究中心,山西太原030031 [6]美国路易斯维尔大学医学院,路易斯维尔40292 [7]中国医学科学院北京协和医学院药物研究所,北京100050

出　　处：《药学学报》2018年第1期147-154,共8页Acta Pharmaceutica Sinica

基　　金：山西省优秀人才科技创新项目(201605D211030);山西省中药产业重点科技攻关项目(201603D311101);国家中药标准化项目(ZYBZH-Y-JIN-34);山西省科技攻关项目(2014ZD0401)

摘　　要：针对目前黄芪商品规格等级无法准确反应生长年限,本研究通过鉴定1~6年恒山仿野生黄芪药材上、下段生长轮数目,获得不同绝对生长年限的黄芪根段,探明其黄酮与皂苷的含量积累规律,为黄芪品质评价奠定基础。采用徒手切片与石蜡切片技术对1~6年生黄芪根中的生长轮现象进行观察研究,分析不同年限黄芪生长轮数目以及同一条根上、下段生长轮数目的差异。利用HPLC-UV-ELSD同时测定方法,对绝对生长2~6年的恒山黄芪样本中12种指标性成分进行分析。结果表明不同年限黄芪根去掉空心部分处生长轮数目与黄芪实际种植年限一致,且同一黄芪根从顶端无空心部分开始至根尖生长轮数目逐渐递减。HPLC-UV-ELSD测定结果表明,3年生皂苷含量最高,4年生黄酮含量达到最大值。研究结果为恒山黄芪药材商品规格等级的划分及临床合理用药提供了依据。Currently, the specification grading standard for Astragali Radix can not accurately reflect growth years. The aim of this study is to identify the growth ring number of different parts of 1 to 6 year Hengshan imitative wild culture Astragali Radix, in order to get a different absolute growth years, to classify the accumulation rules of the content of flavonoids and saponins, and to lay the foundation for evaluating quality of Astragali Radix. Observing growth ring numbers of 1-6 years Astragali Radix by means of hand sections and paraffin sections in the study, and analyzing the number of different growth years and different diameter. At the same time, HPLC-UV-ELSD was used to analyze the 12 index components of the samples with absolute growth years of 2 to 6 years. The results indicated that the growth ring number excepting hollow part is consistent with the actual growth period of Astragali Radix and the number of growth rings gradually decreased from the upper to lower. The results of HPLC-UV-ELSD determination showed that the saponins content of 3-year-old Astragali Radix was the highest while the flavonoids content of the 4-year-old reached the maximum. The study provided the basis for foundation of the specification grading standard for Astragali Radix and clinical rational use drug.

关 键 词：黄芪 生长轮 直径 年限鉴别 等级划分 指标成分 

分 类 号：R931[医药卫生—生药学]