石榴叶提取物诱导前列腺癌细胞凋亡并抑制其增殖转移的研究  

The Extract from Punica Granatum(Pomegranate) Leaves Promotes Apoptosis and Impairs Metastasis in Prostate Cancer Cells

作　　者：邓远乐 李亚丽[1] 郑婷婷[1] 户明星 叶庭洪 谢永美 阴文娅[1] 

机构地区：[1]四川大学华西公共卫生学院营养与食品卫生毒理系,成都610041 [2]四川大学华西医院生物治疗国家重点实验室,成都610041

出　　处：《四川大学学报（医学版）》2018年第1期8-12,共5页Journal of Sichuan University(Medical Sciences)

摘　　要：目的研究石榴叶提取物(pomegranate leaves extract,PLE)对前列腺癌细胞增殖、凋亡及转移能力的影响。方法采用MTT法检测不同质量浓度PLE(终质量浓度分别为12.5、25、50、100、200μg/mL)干预作用不同时间(24、48、72h)对前列腺癌细胞TRAMP-C1、DU145、PC3增殖的影响,克隆形成实验验证PLE对DU145、PC3细胞增殖的长期影响。PLE干预PC3细胞48h后,Hoechst-33258染色观察细胞核内染色质变化,流式细胞术检测细胞凋亡率变化,细胞划痕实验测试细胞迁移运动能力的变化。结果与对照组比较,PLE在12.5~200μg/mL范围内对TRAMP-C1、DU145、PC3细胞增殖具有抑制作用(P<0.05);PLE在6.25~100μg/mL范围内使DU145和PC3集落形成数明显减少(P<0.01)。PLE干预48h后,PC3出现细胞核断裂、产生凋亡小体的现象,随着PLE质量浓度增大,凋亡率逐渐上升(P<0.05),同时PC3细胞向划痕区域迁移生长的能力比对照组低(P<0.01)。结论 PLE能抑制前列腺癌细胞增殖,同时促进PC3细胞凋亡,减弱其迁移能力。Objective To investigate the effects of pomegranate leaves extract （PLE）on proliferation, apoptosis and metastasis of prostate cancer cells. Methods The proliferation of TRAMP-C1, DU145, PC3 prostate cancer cells treated with different concentrations of PLE （final mass concentrations were 12. 5, 25, 50, 100, 200 μg/mL, respectively） for different time （24, 48, 72 h） was detected by MTT assay. Colony formation assay was performed to verify the long-term effects of PLE on the proliferation of DU145 and PC3 cells. After being treated with PLE for 48 h, Hoechst-33258 staining was used to observe the changes in the nucleus, the cell apoptotic rate was detected by flow cytometry, and wound-healing migration assay was perform to test the change of migration. Results In comparison with the control group, PLE in the range of 12. 5-200 μg/mL had a certain inhibitory effect on the proliferation of TRAMP-C1, DU145 and PC3 cells （P〈0. 05）. In the range of 6. 25- 100 μg/mL, the number of colony formation of DU145 and PC3 was significantly reduced （P 〈 0.01 ）. After PLE treated for 48 h,the apoptotic features of nuclear fragmentation and the formation apoptotic body was observed in PC3. With the increase of concentration, the apoptotic rate increased gradually （P〈0. 05）, and the ability of cells to migrate to the scratch area was significantly weaker than the control group （P〈0. 01）. Conclusion PLE has effect on proliferation, apoptosis and metastasis of prostate cancer cells.

关 键 词：石榴叶提取物 前列腺癌 PC3 凋亡 

分 类 号：R285[医药卫生—中药学]