pH法防止转基因蓝藻向环境释放  

pH method to prevent the release of transgenic Anabaena sp.into environment

作　　者：殷嵘 顾娟 施定基[3] 吴倩萍 庄旻敏 何培民[1] 栾必荣 贾睿[1] 

机构地区：[1]上海海洋大学水产与生命学院,上海201306 [2]江苏镇江天和生物技术公司,江苏扬中212200 [3]中国科学院植物研究所,北京100093

出　　处：《生物技术》2017年第6期592-600,共9页Biotechnology

基　　金：国家高技术研究发展计划(863计划)("阻断白斑综合征病毒WSSV感染对虾的VP28蛋白研发";No.2014AA 093506);上海市科技兴农推广项目("对虾白斑综合征病毒的重组蓝藻疫苗研发与应用";沪农科推字(2017)第1-13号);上海市科委项目("对虾白斑综合症病毒的重组蓝藻疫苗研发";No.16391903500;"防治对虾病毒的易检测转基因蓝藻口服剂研制";No.17391902200)

摘　　要：[目的]防止转基因蓝藻(Anabaena sp.PPC 7120)在制备对虾抗白斑综合征病毒口服药过程中向环境释放,保证制药安全性。[方法]用不同pH处理转基因蓝藻,并在不同时间、温度和光照下观察效果,并用响应面法优化处理条件。[结果]最佳处理条件为:pH≤2.5、t≥50 min、T≥30℃或pH≥12、t≥120 min、T≥30℃,杀藻率为100%。两个优化组合分别为:pH 2.12、温度29.96℃、时间33.9 min或pH 12.33、温度31.75℃、时间96.8 min。pH处理后的转基因蓝藻在平板上不能再继续生长。[结论]pH法对转基因蓝藻的杀藻率可达100%,有效防止了转基因蓝藻在制备对虾口服剂过程中发生环境释放。[Obiective]The release of Anabaena sp.PPC 7120 into environment during the preparation of the oral shrimp drug would be stopped,and pharmaceutical safety would be ensured.[Methods] The transgenic Anabaena sp.was treated with different pH and observed at different times,temperatures and illuminations.The response surface method was used to optimize the treatment conditions.[Results]The optimum treatment conditions were as follows： pH≤2.5,t≥50 min,T≥30 ℃ or pH≥12,t≥120 min,T ≥ 30 ℃,and the killed cell rate is up to 100%.The two optimal combinations were as follows： pH 2.12,29.96 ℃,33.9 min or pH 12.33,31.75 ℃,96.8 min.After the treatment with pH,the transgenic Anabaena sp.was not able to continue to grow on the plate completely.[Conclusion] The killed cell rate of the pH method up to 100% which can effectively prevent the release into environment of transgenic Anabaena sp.during the preparation of the oral shrimp drug.

关 键 词：PH 杀藻率 转基因蓝藻 环境释放 重组药物安全性 响应面法 重组药物制备 

分 类 号：Q939.99[生物学—微生物学]