蒌叶抑菌活性成分的超声提取及其稳定性的研究  

Study on ultrasonic-assisted extraction and stability of antibacterial components from Piper betle L.

作　　者：吕纪行[1] 纪明慧[2] 郭飞燕[2] 何猛雄[2] 吴香金 

机构地区：[1]南京大学化学化工学院,江苏南京210023 [2]海南师范大学化学与化工学院,海南海口571158

出　　处：《化学研究与应用》2018年第1期46-53,共8页Chemical Research and Application

基　　金：海南省社会发展科技专项(2015SF48)资助

摘　　要：以超声波提取法对蒌叶中抑菌活性成分进行提取,考察不同溶剂、料液比、提取时间、提取温度、提取功率对提取工艺的影响,同时采用96孔板法和倾注平板法测定了提取物对枯草杆菌、金黄色葡萄球菌、大肠杆菌、毛霉、黑曲霉、青霉的最小抑菌浓度;并考察了温度、光照、放置时间对提取物抑菌活性稳定性的影响。结果表明:蒌叶抑菌活性成分提取的最佳工艺条件为,无水乙醇为溶剂、m_(蒌叶干粉)∶V_(乙醇)=1.75∶10(g·mL^(-1))、提取时间为60 min、提取功率为192 W、提取温度为50℃时,提取物的抑菌效果最好。对大肠杆菌、枯草杆菌、金黄色葡萄球菌、黑曲霉、毛霉、青霉最小抑菌浓度分别为5.00、2.50、2.50、1.25、1.25、2.50 mg·mL^(-1)。同时,提取物的石油醚部位和氯仿部位富集有抑菌活性很强的物质。在设定的温度范围、光照条件下,蒌叶提取物的抑菌活性较稳定。因此,蒌叶乙醇提取物的抑菌活性具有一定的光热稳定性。The Piper betle was extracted by ultrasonic extraction, and the influence of solvent type, solid- to-Iiquid ratio, extraction time, extraction temperature and power on the extraction yields of compounds tested was investigated. To study antibacterial activities of the extracts from the Piper betle, usingthe 96-well plates and pour plat emethod. Meanwhile, different temperature, light and time affecting the antibacterial activity of the extracts were also investigated. The results indicated that the optimal conditions were anhy-drous ethanol as extraction solvent, solid- to-Iiquid ratio 1.75 ： 10（g ·mL^-1） , ultrasonic time 60 min, ultrasonic power192W , ex-traction temperature 50℃. The extracts had certain antibacterial activity on the microorganisms,under the optimized conditions,MIC for escherichia col i, bacillus subtilis,staphylococcus aureus,aspergillus nigar, mucor,penicllilum notatumwas5. 00、2. 50、2. 50、 1. 25、1. 25、2. 50 mg·mL^-1 respectively. Meanwhile,there are antibacterial components in the petroleum ether extraction and chlor-oform extraction. The extract showed well stability on different temperature and light what were set up. Therefore, the extract has def- inites tability on different temperature and light.

关 键 词：蒌叶 抑菌成分 超声波辅助提取法 稳定性 

分 类 号：O658.2[理学—分析化学]