孕晚期血清25-OH-VitD3含量与母体内皮损伤、胎盘细胞凋亡的相关性  

Correlation of late-pregnancy serum25-OH-VitD3 content with maternal endothelial injury and placental apoptosis

作　　者：张凡 陈爱华[1] 

机构地区：[1]三峡大学人民医院&宜昌市第一人民医院妇产科,湖北宜昌443000

出　　处：《海南医学院学报》2018年第1期49-51,55,共4页Journal of Hainan Medical University

基　　金：宜昌市医疗卫生科技计划项目(A01322-09)~~

摘　　要：目的:研究子痫前期(PE)孕妇孕晚期血清25-OH-VitD3含量与母体内皮损伤、胎盘细胞凋亡的相关性。方法:选择在宜昌市第一人民医院分娩的子痫前期孕妇和健康孕妇,分别作为PE组和对照组。孕32周及分娩前,采集血清并测定25-OH-VitD3及内皮损伤标志物的含量;分娩后,采集胎盘并测定细胞凋亡分子的表达量。结果:PE组孕妇血清中25-OH-VitD3的含量以及胎盘组织中XIAP、Survivin的mRNA表达量显著低于对照组,血清中sEng、sFlt-1、IFI16、tTG的含量以及胎盘组织中AP-2α、Smac、PTEN的mRNA表达量显著高于对照组;25-OH-VitD3低含量PE孕妇血清中sEng、sFlt-1、IFI16、tTG的含量以及胎盘组织中AP-2α、Smac、PTEN的mRNA表达量显著高于25-OHVitD3高含量PE孕妇,XIAP、Survivin的mRNA表达量显著低于25-OH-VitD3高含量PE孕妇。结论:PE孕妇孕晚期血清中25-OH-VitD3含量的降低能够加重母体内皮损伤、胎盘细胞凋亡。Objective:To study the correlation of late-pregnancy serum 25-OH-VitD3 content with maternal endothelial injury and placental apoptosis in pregnant women with preeclampsia(PE).Methods:Preeclampsia pregnant women and healthy pregnant women who gave birth in the First People's Hospital of Yichang between June 2014 and February 2017 were selected as PE group and control group respectively.At 32 weeks of gestation and before delivery,the serum was collected respectively to determine the contents of 25-OH-VitD3 and endothelial lesion markers;after delivery,the placenta was collected to determine the expression of apoptosis molecules.Results:25-OH-VitD3 contents in serum as well as XIAP and Survivin mRNA expression in placental tissue of PE group were significantly lower than those of control group while sEng,sFlt-1,IFI16 and tTG contents in serum as well as AP-2α,Smac and PTEN mRNA expression in placental tissue were significantly higher than those of control group;sEng,sFlt-1,IFI16 and tTG contents in serum as well as AP-2α,Smac and PTEN mRNA expression in placental tissue of PE pregnant women with low 25-OH-VitD3 content were significantly higher than those of PE pregnant women with high 25-OH-VitD3 content while XIAP and Survivin mRNA expression were significantly lower than those of PE pregnant women with high 25-OH-VitD3 content.Conclusion:The decline of late-pregnancy serum 25-OH-VitD3 content can aggravate the maternal endothelial injury and placental apoptosis in pregnant women with PE.

关 键 词：子痫前期 25-羟维生素D3 内皮损伤 细胞凋亡 

分 类 号：R714.245[医药卫生—妇产科学]