一种酵母表达载体的构建及表达鉴定  

Construction and identification of a saccharomyces cerevisiae recombinant expression vector

作　　者：王海涛[1] 李雪萍[1] 郭红云[1] 王涛[1] 闵建平[1] 郭欢[1] 朱公建[1] 苏海翔[1] 

机构地区：[1]甘肃省医学科学研究院,甘肃兰州730050

出　　处：《甘肃医药》2017年第12期993-996,共4页Gansu Medical Journal

基　　金：甘肃省青年科技基金计划项目(项目编号:1208RJYA072)

摘　　要：目的:通过基因工程技术构建霍乱毒素B亚基(CTB)与人Aβ42的融合基因,并进行表达鉴定。方法:将融合基因克隆到酵母表达载体pYES2中,并将鉴定正确的重组载体通过乙酸锂转化法转入野生型酵母菌株INVSc1。筛选阳性转化株,并将其诱导表达,通过Western blot检测融合蛋白CTB-Aβ42。结果:成功构建了CTB-Aβ42融合基因的酵母表达载体,并且可以有效表达融合蛋白。结论:本研究为进一步研制有效的抗阿尔茨海默症药物提供了一种新思路,具有一定的理论和应用价值。Objective:Design the fusion gene of CTB and Aβ42 by genetic engineering technologies,and detect its expression.Methods:Cloned the fusion gene into Saccharomyces cerevisiae expression vector pYES2. The recombinant plasmid was transfected into Saccharomyces cerevisiae INVSc1 using lithium acetate and the expression of fusion protein was tested by western blot. Results:The Saccharomyces cerevisiae recombinant expression vector was constructed successfully and the fusion protein of CTB and Aβ42 was expressed effectively. Conclusion:This study provided a new thought for further investigation of an effective anti-Alzheimer's disease drug. It has important value of theory and application.

关 键 词：乱毒素B亚基 茁淀粉样蛋白 融合蛋白 酵母表达 

分 类 号：Q78[生物学—分子生物学]