TRUST和ELISA在梅毒检测中的应用  

Application of TRUST and ELISA in the Detection of Syphilis

作　　者：尤家伟[1] 祝峰[1] 周涛 卢丹 许璐 祝靓 

机构地区：[1]武警江苏省总队医院检验科,江苏扬州225003

出　　处：《医疗装备》2018年第1期79-80,共2页Medical Equipment

摘　　要：目的比较梅毒螺旋体检测中甲苯胺红不加热血清试验(TRUST)和酶联免疫吸附试验(ELISA)的灵敏度、特异度及其结果。方法用TRUST和ELISA分别对280例梅毒确诊患者血清标本以及188例健康人群血清标本进行检测。结果 468例被检血清标本中,TRUST阳性143例,阳性率30.56%;ELISA阳性277例,阳性率59.19%。TRUST灵敏度50.4%,特异度93.6%;ELISA灵敏度98.9%,特异度100.0%。结论TRUST检测的是梅毒非特异性抗体,ELISA检测的是梅毒特异性抗体,临床上筛查梅毒应结合两种方法,有效地减少漏诊和误诊。Objective To compare the specificity, sensitivity and application value of the TRUST and ELISA commonly used in clinical tests of syphilis. MethodsApply the TRUST and ELISA methods to 280 serum samples from syphilis patients and 188 serum samples from control group. ResultsIn 468 serum samples, the numbers of positive samples of TRUST and ELISA are 143 and 277. The positive rates of TRUST and ELISA are 30.5% and 59.2%. The sensitivity and specificity of TRUST are 50.4% and 93.6%. The sensitivity and specificity of ELISA are 98.9% and 100%. ConclusionTRUST detects Non-specific antibody of syphilis. ELISA detects specific antibody of syphilis. They should be used together to screening syphilis, to reduce the rate of missed diagnosis and misdiagnose.

关 键 词：梅毒 甲苯胺红不加热血清试验 酶联免疫吸附试验 

分 类 号：R446.6[医药卫生—诊断学]